Relationship of the mitogen-activated protein kinase with skeletal muscle fiber size and the changes of fiber type composition

• Project/Area Number: 15K01620
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Sports science
• Research Institution: Kumamoto University
• Principal Investigator: Oishi Yasuharu 熊本大学, 教育学部, 教授 (10203704)
• Research Collaborator: Roy Roland R. University of California Los Angeles, Department of Integrative Biology and Physiology and Brain Research Institute
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ラット骨格筋 / MAPK / ERK1/2 / 徐神経 / ヒラメ筋 / 足底筋 / 腓腹筋 / リン酸化 / 発育 / マップキナーゼ / 筋線維組成 / リン酸化マップキナーゼ / ラット / Heat shock protein

Outline of Final Research Achievements

The purpose of the present study is to clarify the muscle fiber phenotype specificity of 42/44 kDa MAPK (ERK1/2) protein. Expression level of phosphorylated ERK 1/2 was analyzed for the 10-wk-old　Wistar　rat　and　the　level　was　hoghest　in　the　superficial　portion　of　the　gastrocnemius muscle, lower in the plantaris and deep gastrocnemius, and lowest in the soleus muscle. This order is the same with the ratio of fast type fiber containing in each muscle. Rat soleus muscle contained the fast type fiber highest in the 3-wk-old, and　gradually　decrease　during　growing　and　aging　stages.　Similar　change　was　observed　in　the　expression level of phosphorylated ERK1/2. In addition, the increase in the fast-phenotype fiber and the phosphorylated ERK1/2 was observed in the denervated rat soleus muscle.
These results strongly suggest that the expression of phosphorylated ERK1/2 is closely related to that of the fast phenotype fiber in rat skeletal muscles.

Research Products

• [Int'l Joint Research] Brain Research Institute/University of California Los Angeles(米国)
• [Int'l Joint Research] university of California Los Angeles(米国)
• [Journal Article] Heat-stress effects on the myosin heavy chain phenotype of rat soleus fibers during the early stages of regeneration (2015)
  • Author(s): Oishi Y, Roy RR, Ogata T, Ohira Y.
  • Journal Title: Muscle & Nerve Volume: 52 Issue: 6 Pages: 1047-1056
  • DOI: 10.1002/mus.24686
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] The level of 44/42 kDa MAPK protein is closely related to the fiber type composition in hindlimb skeletal muscle of sedentary adult rats and in the soleus muscle during development (2016)
  • Author(s): Oishi Y, Ogata T, Roy RR
  • Organizer: European College of Sports Science
  • Place of Presentation: Austria
  • Year and Date: 2016-07-06
  • Int'l Joint Research
• [Presentation] The level of 44/42 kDa MAPK protein is closely related to the fiber type composition in hindlimb skeletal muscles of sedentary adult rats and in the soleus muscle during development (2016)
  • Author(s): Oishi Y, Ogata T, Ohira Y, Roy RR.
  • Organizer: European College of Sports Sciences
  • Place of Presentation: Austria
  • Year and Date: 2016-07-06
  • Int'l Joint Research