| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The purpose of the present study is to clarify the muscle fiber phenotype specificity of 42/44 kDa MAPK (ERK1/2) protein. Expression level of phosphorylated ERK 1/2 was analyzed for the 10-wk-old Wistar rat and the level was hoghest in the superficial portion of the gastrocnemius muscle, lower in the plantaris and deep gastrocnemius, and lowest in the soleus muscle. This order is the same with the ratio of fast type fiber containing in each muscle. Rat soleus muscle contained the fast type fiber highest in the 3-wk-old, and gradually decrease during growing and aging stages. Similar change was observed in the expression level of phosphorylated ERK1/2. In addition, the increase in the fast-phenotype fiber and the phosphorylated ERK1/2 was observed in the denervated rat soleus muscle.
These results strongly suggest that the expression of phosphorylated ERK1/2 is closely related to that of the fast phenotype fiber in rat skeletal muscles.
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