Effects of TGF-beta-TIMP1 pathway on white adipose tissue remodeling by obesity and exercise
| Project/Area Number |
15K01626
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Sports science
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 脂肪組織 / 肥満 / 運動トレーニング / リモデリング / 細胞外マトリックス / TGF-β / TIMP1 |
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| Outline of Final Research Achievements |
In the present study, we examined effects of TGF-β and TIMP1 on WAT remodeling by exercise training (TR) and obesity. The mRNA levels of TGF-β and TIMP1 in WAT of obese mice were significantly higher than those in WAT of control mice, and TR significantly attenuated the expression of these genes. The gene expression and the protein production of TIMP1 in 3T3-L1 cells were upregulated by treatment with TGF-β. Moreover, treatment of 3T3-L1 cells with TIMP1 resulted in attenuation of insulin-induced glucose uptake and phosphorylation of Akt. In addition, the reduction of prothymosin α in 3T3-L1 cells resulted in enhanced gene expressions of TIMPs (TIMP1-3) and TGF-β signaling related factors, such as dermatopontin. These results suggest that, TR improves insulin sensitivity by inhibiting the TGF-β-TIMP1 pathway associated with reduction of glucose uptake in WAT.
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Report
(4 results)
Research Products
(49 results)
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[Journal Article] Cerebral artery blood flow and oxygenation in the frontal lobe region in response to a judo chokehold (shimewaza)2016
Author(s)
Haga S, Sakurai T, Hamaoka T, Esaki K, Ueya K, Toshinai K, Miyazaki H, Ogasawara J, Shirato K, Hashimoto N, Kastumura T, Nioka S, Chance B, Yamaguchi I, Kizaki T, Ohno H
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Journal Title
Journal of Exercise, Sports & Orthopedics
Volume: 3
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Ghrelin augments the expressions and secretions of proinflammatory adipokines, VEGF120 and MCP-1, in differentiated 3T3-L1 adipocytes.2015
Author(s)
A. Kitahara, K. Takahashi, R. Moriya, H. Onuma, K. Handa, Y. Sumitani, T. Tanaka, H. Katsuta, S. Nishida, T. Sakurai, K. Inukai, H. Ohno, and H. Ishida.
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Journal Title
J Cell Physiol
Volume: 230
Issue: 1
Pages: 199-209
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] The TGF-β-TIMP1 pathway, which inhibits glucose uptake in adipocytes, is attenuated by exercise training2015
Author(s)
Sakurai T, Ogasawara J, Shirato K, Ishibashi Y, Ozawa T, Ohira Y, Imaizumi K, Ohno H, Kizaki T
Organizer
The 16th International Biochemistry of Exercise Conference (IBEC 2015)
Place of Presentation
Sao Paulo, Brazil
Year and Date
2015-09-07
Related Report
Int'l Joint Research
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[Presentation] Voluntary exercise reduces O-linked N-acetylglucosamine levels in murine macrophages2015
Author(s)
Shirato K, Sakurai Y, Ogasawara J, Ishibashi Y, Kato H, Izawa T, Ohira Y, Imaizumi K, Ohno H, Kizaki T
Organizer
The 16th International Biochemistry of Exercise Conference (IBEC 2015)
Place of Presentation
Sao Paulo, Brazil
Year and Date
2015-09-07
Related Report
Int'l Joint Research
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