The role of GAPDH nitration in insulin signaling
| Project/Area Number |
15K01728
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | Juntendo University |
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Principal Investigator |
BABA Takeshi 順天堂大学, 医学部, 准教授 (80366450)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 心筋 / インスリン / 翻訳後修飾 / 2型糖尿病 |
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| Outline of Final Research Achievements |
GAPDH is a catalytic enzyme commonly known to be involved in glycolysis. Increase in GAPDH nitration as well as phosphorylation was observed after insulin stimulation in the H9c2 cell line derived from embryonic rat ventricle. After glucose intraperitoneal injection, the phosphorylation and nitration levels of GAPDH were weak in cardiac muscle of type 2 diabetic rat compared with normal rat. These results demonstrated the possibility that insulin signaling mediated by GAPDH was impaired in the heart of type 2 diabetic rat. GAPDH was nitrated on Trp 311 after insulin stimulation of the H9c2 cell line. We mutated Trp 311 of GAPDH to Phe to prove that the nitration of Trp 311 acts as a trigger for the phosphorylation of GAPDH. The phosphorylation of the W/F GAPDH mutant diminished considerably compared to that of wildtype GAPDH after insulin stimulation in H9c2 cell line. These data suggest that the nitration of GAPDH may play a role in the insulin signal transduction in cardiac muscle.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Accumulation of immunoglobulin G against Dermatophagoides farinae tropomyosin in dorsal root ganglia of NC/Nga mice with atopic dermatitis-like symptoms.2017
Author(s)
Otsu A, Kawasaki H, Tominaga M, Shigenaga A, Matsuda H, Takahashi N, Nakajima T, Naito H, Baba T, Ogawa H, Tomooka Y, Yamakura F, Takamori K.
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Journal Title
Biochem Biophys Res Commun.
Volume: 485
Issue: 4
Pages: 707-712
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Localization of IgG against D. farina-tropomyosin in dorsal root ganglia of NC/Nga mice with atopic dermatitis-like symptoms2016
Author(s)
Otsu A, Kawasaki H, Tominaga M, Shigenaga A, Iizumi K, Baba T, Naito H, Ogawa H, Nakajima T, Tomooka Y, Yamakura F, Takamori K
Organizer
The 41st Annual Meeting of The Japanese Society for Investigative Dermatology
Place of Presentation
Sendai International Center
Year and Date
2016-12-09
Related Report
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