| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Expression analysis was performed on fat-soluble ligand receptor (GPCR) expressed in skeletal muscle by RT-PCR method in skeletal muscle model cell C2C12. We examined the relationship between expression of GPR30 and GPR81 in muscle fiber differentiation in C2C12 and muscle satellite cells (SC) and the sensitivity of estrogen and lactic acid to muscle fiber differentiation. We further investigated the regulation of signaling pathway from GPR30 and GPR81.
We also developed a skeletal muscle fiber differentiation model in vitro to construct a skeletal muscle model near the biological system. Furthermore, we constructed a co-culture system of SC and skeletal muscle cells, and could observe the process of differentiation into muscle fibers.
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