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Molecular mechanisms of the cellular signaling by which inorganic polyphosphate promotes calcification on osteoblastic cell

Research Project

Project/Area Number 15K01793
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomolecular chemistry
Research InstitutionHokkaido University

Principal Investigator

Tsutsumi Kaori  北海道大学, 保健科学研究院, 助教 (80344505)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsポリリン酸 / 石灰化 / ATP / ミトコンドリア / 骨芽細胞 / ヌクレオチド代謝
Outline of Final Research Achievements

Inorganic polyphosphate (polyP) is a linear polymer containing tens to hundreds of orthophosphate residues and found in all living organisms from bacteria to mammals. In the present study, we examined the molecular mechanism of polyP to promote calcification on MC3T3-E1 osteoblastic cells. Cells cultured with polyP were strongly stained with Alizarin red. Long-chain length of polyP was the most effective chain length. PolyP-treated cells induced the depletion of cellular ATP contents accompany with ADP conversion though treatment with polyP did not affect cell growth. In the polyP-treated cells, the mitochondria was swollen with low dense observed in electron microscopy. Mitochondrial membrane potential was significantly decreased in polyP-treated cells. These results suggested that the depletion of intracellular ATP and the decrease in mitochondrial membrane potential by polyP treatment would be a trigger to promote a cellular calcification.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (2 results)

All 2018 2015

All Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] PolyP promotes cellular calcification in association with ATP depletion and mitochondrial swelling2018

    • Author(s)
      Kaori Tsutsumi, Tatsuya Sasase, Nagahito Saito, Toshikazu Shiba
    • Organizer
      9th World Congress on Targeting Mitochondria
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Involvement of inorganic polyphosphate in radiosensitivity of human non-small cell lung cancer cell line2015

    • Author(s)
      堤 香織
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸国際会議場(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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