| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Ladder-shaped polyethers (LSPs) are secondary metabolites produced by marine dinoflagellates and have unique structures and strong bioactivities. The biosynthesis of LSPs has been proposed. However, the fused ether ring formation of marine LSPs has not yet been elucidated because of a lack of genetic information of dinoflagellates and the complexity of these structures.
For exploration of biosynthetic enzymes such as epoxidase and an epoxide-opening enzyme, model precursor compounds were synthesized. Incubation of the model compounds with dinoflagellate extracts gave rise to a 6-membered ether ring products. These results strongly indicated that the epoxide-opening enzyme was found in the extract from dinoflagellates.
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