Lipid interaction and headgroup conformation of glycosphingolipids in membrane bilayer
| Project/Area Number |
15K01801
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 糖脂質 / 生体膜 / 構造 / NMR / 合成 / ガングリオシド / コレステロール / 顕微鏡 / リポソーム / 脂質ラフト / スフィンゴ糖脂質 / DSC |
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| Outline of Final Research Achievements |
Glycosphingolipids (GSLs) are the membrane lipid molecules having common ceramide backbone and oligosaccharide headgroups with various size and charge. GSLs have been thought to interact with cholesterol (Cho) and altered the sugar chain conformation to regulate the protein interactions, but detail was elusive. Here, we used biomimetic membranes to analyze GSL-Cho interaction and the headgroup conformation by solid state NMR and fluorescence spectroscopies. Lactosylceramide was found to form rigid cluster through headgroup hydrogen bonding, and the affinity to Cho was weak. Glucosylceramide received some order effect from Cho, but the headgroup conformation was not significantly altered in the presence of Cho.
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Report
(4 results)
Research Products
(22 results)
