Structure and function of a signal protein osteocalcin connncting bone metabolism and carbohydrate metabollism

• Project/Area Number: 15K01805
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomolecular chemistry
• Research Institution: Chitose Institute of Science and Technology
• Principal Investigator: Kawano Keiichi 千歳科学技術大学, 理工学部, 特別研究員 (10136492)
• Co-Investigator(Renkei-kenkyūsha): Karthaus Olaf 千歳科学技術大学, 理工学部, 教授 (80261353) ; KIMURA Hiromi 千歳科学技術大学, 理工学部, 教授 (00574857)
• Research Collaborator: KOBAYASHI Shota ; AIZAWA Tomoyasu ; MIZUGUCHI Mineyuki ; YOKOYAMA Takeshi ; NABESHIMA Yuko ; HIRATA Masato
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: オステオカルシン / 多臓器相関 / 糖代謝 / 骨形成 / 立体構造解析 / NMR / X線結晶解析 / 活性型 / ハイドロキシアパタイト / 吸着 / ＮＭＲ / Ｘ線結晶解析 / 安定同位体標識

Outline of Final Research Achievements

Osteocalcin (OC), which was known as a protein useful for the formation of bone, has been found to be a hormone with various activities such as control of sugar metabolism, lipid metabolism, production of testosterone. Stimulation of bones is known as a key of health and rejuvenation. Despite its importance, a little of molecular mechanisms are known. Since OC is activated in an acidic environment, the three-dimensional structures of OC in acidic state were examined by CD, NMR and X-ray crystallography. As a result, it was revealed that OC is able to maintain structure and hormonal activity even in acidic environment.

Research Products

• [Journal Article] Color changes of a full-color emissive ESIPT fluorophore in response to recognition of certain acids and their conjugate base anions (2018)
  • Author(s): S. Tsuchiya, K. Sakai, K. Kawano, Y. Nakane, T. Kikuchi, T. Akutagawa
  • Journal Title: Chem. Eur. J. Volume: 24 Issue: 22 Pages: 5868-5875
  • DOI: 10.1002/chem.201705622
  • NAID: 120006650285
  • Peer Reviewed
• [Journal Article] Conformation of Carboxylated and Uncarboxylated Osteocalcin in the Bone Resorbing Niche. (2017)
  • Author(s): Shota Kobayashi, Keiichi Kawano, Tomoyasu Aizawa, Akiko Mizokami, Olaf Karthaus, and Masato Hirata
  • Journal Title: Peptide Science 2016 Volume: 1 Pages: 79-80
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Lipopolysaccharide-bound structure of the antimicrobial peptide cecropin P1 determined by nuclear magnetic resonance spectroscopy. (2016)
  • Author(s): Baek MH, Kamiya M, Kushibiki T, Nakazumi T, Tomisawa S, Abe C, Kumaki Y, Kikukawa T, Demura M, Kawano K, Aizawa T.
  • Journal Title: J Pept Sci. Volume: 22 Issue: 4 Pages: 214-221
  • DOI: 10.1002/psc.2865
  • NAID: 120005981524
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] In vivo fluorescence correlation spectroscopy analyses of FMBP-1, a silkworm transcription factor (2016)
  • Author(s): Motosuke Tsutsumi, Hideki Muto, Shohei Myoba, Mai Kimoto, Akira Kitamura, Masakatsu Kamiya, Takashi Kikukawa, Shigeharu Takiya, Makoto Demura, Keiichi Kawano, Masataka Kinjo, Tomoyasu Aizawa
  • Journal Title: FEBS openbio Volume: 6 Issue: 2 Pages: 106-125
  • DOI: 10.1002/2211-5463.12026
  • NAID: 120005756103
  • Peer Reviewed / Open Access
• [Journal Article] Solution structure of carboxylated and uncarboxylated osteocalcin. (2016)
  • Author(s): Keiichi Kawano, Shota Kobayashi, Tomoyasu Aizawa, Olaf Karthaus and Masato Hirata
  • Journal Title: Peptide Science 2015 Volume: 1 Pages: 141-142
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Efficient production of a correctly folded mouse α-defensin, cryptdin-4, by refolding during inclusion body solubilization. (2015)
  • Author(s): Tomisawa S, Sato Y, Kamiya M, Kumaki Y, Kikukawa T, Kawano K, Demura M, Nakamura K, Ayabe T, Aizawa T.
  • Journal Title: Protein Expr Purif. Volume: 112 Pages: 21-28
  • DOI: 10.1016/j.pep.2015.04.007
  • NAID: 120005820696
  • Peer Reviewed / Open Access
• [Presentation] Conformation of Carboxylated and Uncarboxylated Osteocalcin in Acidic Condition. (2016)
  • Author(s): Shota Kobayashi, Keiichi Kawano, Tomoyasu Aizawa, Akiko Mizokami, Olaf Karthaus, and Masato Hirata
  • Organizer: 17th Chitose International Forum on Photonics Science and Technology
  • Place of Presentation: 千歳科学技術大学（北海道千歳市）
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Conformation of Carboxylated and Uncarboxylated Osteocalcin in the Bone Resorbing Niche. (2016)
  • Author(s): Shota Kobayashi, Keiichi Kawano, Tomoyasu Aizawa, Akiko Mizokami, Olaf Karthaus, and Masato Hirata
  • Organizer: 第53回ペプチド討論会
  • Place of Presentation: 京都テルサ（京都府京都市）
  • Year and Date: 2016-10-26
• [Presentation] Solution structure of carboxylated and uncarboxylated osteocalcin. (2015)
  • Author(s): Keiichi Kawano, Shota Kobayashi, Tomoyasu Aizawa, Olaf Karthaus and Masato Hirata
  • Organizer: 第52回ペプチド討論会
  • Place of Presentation: 平塚中央公民館（神奈川県平塚市）
  • Year and Date: 2015-11-16