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Biochemical analysis of Hybrid type polyketide synthase Steely that produce multi-products

Research Project

Project/Area Number 15K01807
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomolecular chemistry
Research InstitutionSophia University

Principal Investigator

SAITO Tamao  上智大学, 理工学部, 教授 (30281843)

Co-Investigator(Kenkyū-buntansha) 森田 直樹  国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ付 (60371085)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsポリケタイド / 生合成 / 微生物
Outline of Final Research Achievements

Polyketide synthase “Steely” has a distinct structure that is a fusion of a type I and III polyketide synthases. One of them, SteelyB was identified to be responsible for the production of DIF-1 that induces (pre)stalk cells differentiation in the slug stage of D. discoideum development. We identified LCC-1, chlorinated benzofuran that accumulated in the stalk region of fruiting bodies as second product of SteelyB enzyme. NMR and Crystal structural analysis revealed the detailed structure of LCC-1. The result of chlorine labelling experiment showed that ChlA, a chlorinating enzyme that regulated DIF-1 production was also involved in biosynthetic pathway of LCC-1. The feeding experiment indicated that THPH, a DIF-1 polyketide backbone rescued the production of LCC-1 in stlB null mutant. From these results, we hypothesized that SteelyB and ChlA are also involved in the LCC-1 biosynthesis. These two enzymes change their localization and thus change the modifying enzymes to produce LCC-1

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2018 2017 2016

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results)

  • [Journal Article] Stalk cell differentiation without polyketides in the cellular slime mold.2016

    • Author(s)
      Yukie G. Sato, Teresa Suarez & Tamao Saito
    • Journal Title

      Biosci. Biotechnol. Biochem.,

      Volume: 80 Issue: 7 Pages: 1368-1374

    • DOI

      10.1080/09168451.2016.1162087

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 細胞性粘菌が生産するハロゲン化有機化合物の機能と生合成機構の解析2018

    • Author(s)
      飯島知之、品川知則、臼杵豊展、鈴木教之、Kay RR、齊藤玉緒
    • Organizer
      2018 年度日本農芸化学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 細胞性粘菌Dictyostelium discoideumの細胞集合と胞子成熟の機能経路に関する研究2017

    • Author(s)
      近藤杏奈、成田隆明、村田ちひろ、臼杵豊展、齊藤玉緒
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府・京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] ハイブリッド型ポリケタイド合成酵素SteelyBの産物多様性機構の解析2016

    • Author(s)
      飯島知之、品川知則、深澤汐香、臼杵豊展、鈴木教之、齊藤玉緒
    • Organizer
      第29回植物脂質シンポジウム
    • Place of Presentation
      大阪大学(大阪府・豊中市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
  • [Presentation] ハイブリッド型ポリケタイド合成酵素SteelyBの産物多様性の解析2016

    • Author(s)
      飯島知之、齊藤玉緒
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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