| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Polyketide synthase “Steely” has a distinct structure that is a fusion of a type I and III polyketide synthases. One of them, SteelyB was identified to be responsible for the production of DIF-1 that induces (pre)stalk cells differentiation in the slug stage of D. discoideum development. We identified LCC-1, chlorinated benzofuran that accumulated in the stalk region of fruiting bodies as second product of SteelyB enzyme. NMR and Crystal structural analysis revealed the detailed structure of LCC-1. The result of chlorine labelling experiment showed that ChlA, a chlorinating enzyme that regulated DIF-1 production was also involved in biosynthetic pathway of LCC-1. The feeding experiment indicated that THPH, a DIF-1 polyketide backbone rescued the production of LCC-1 in stlB null mutant. From these results, we hypothesized that SteelyB and ChlA are also involved in the LCC-1 biosynthesis. These two enzymes change their localization and thus change the modifying enzymes to produce LCC-1
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