| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
This research aims to develop a tool to control protein transport and localization by utilizing the ligand-inducible －1 ribosomal frameshifting (－1PRF). －1PRF is a recoding mechanism to make alternative proteins from a single mRNA transcript. －1PRF is stimulated by cis-acting signals in mRNA, a slippery sequence and a downstream pseudoknot. In this study we engineered the frameshifting pseudoknot to respond to our designed small molecule naphthyridine carbamate tetramer (NCTn). We demonstrate that NCTn can stabilize the pseudoknot structure in mRNA and activate －1PRF, thereby induce synthesis of a protein from an alternative reading frame in cells. The ligand-inducible －1PRF system developed in this study provides a new tool for de novo production of a fusion protein by small synthetic molecules, and therefore can be applied to cellular transport and localization of target proteins in cells.
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