Synthetic study of inner-core oligosaccharide of LPS/LOS recognized by human antibody
| Project/Area Number |
15K01822
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | リポ多糖 / コアオリゴ糖 / 立体選択的合成 / Kdo / Heptose / 糖鎖プローブ / 抗体 / LPS / core oligosaccharide / synthesis / library / グリコシル化 / リポ多糖(LPS) / 糖鎖ライブラリ / 分岐糖合成 / 糖鎖合成 / コア糖鎖 / 免疫刺激 |
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| Outline of Final Research Achievements |
The synthesis of continuously branched core hexasaccharide produced by gram-negative bacteria was successfully accomplished by using block coupling of the oligosaccharide donor with a 2-4 linked Kdo acceptor. For the construction of 3,4-branched heptose structure, the use of toluene was found to be effective solvent from a 4-O-substituted heptose with a heptose donor. The 4,5-branched tri-, tetra-, and pentasaccharide were also synthesized with complete stereoselectivity by the same manner. The oligosaccharide-biotin conjugate and sugar coated magnetic nanoparticle were synthesized. The human IgG selected by using the synthetic carbohydrate coated magnetic nanoparticle was found to recognized Salmonella minesota Re-LPS.
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Report
(4 results)
Research Products
(19 results)
