Cell specific manipulation of plant hormone responses

• Project/Area Number: 15K01828
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Chemical biology
• Research Institution: Okayama University of Science
• Principal Investigator: Hayashi Ken-ichiro 岡山理科大学, 理学部, 教授 (30289136)
• Co-Investigator(Kenkyū-buntansha): 三井 亮司 岡山理科大学, 理学部, 教授 (60319936)
• Co-Investigator(Renkei-kenkyūsha): Kasahara Hiroyuki 東京農工大学, 農学部, 教授 (00342767)
• Research Collaborator: Zhao Yunde カルフォルニア大学, サンディエゴ校, 教授
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: オーキシン / ケミカルバイオロジー / 植物ホルモン / 植物 / ドラッグデリバリー

Outline of Final Research Achievements

Asymmetric auxin distribution plays a crucial role in plant growth and development to adapt the environmental stimulus. The local biosynthesis of IAA and the inactivation and degradation of IAA would also contribute to the modulation of auxin distribution.　We present that new auxin application system using inactive auxin-prodrug and its metabolic activation enzyme. The IAA-prodrug activating enzyme (IAP) was cloned and expressed as GFP-IAP fusion protein in E. coli and Arabidopsis thaliana. Transgenic plant expressing GFP-IAP showed auxin-induced phenotypes when IAA-prodrug was applied. This new tools would be useful tools to dissect the spatiotemporal dynamics of IAA distribution leading to cell-specific auxin responses.

Research Products

• [Int'l Joint Research] UC San Diego(米国)
• [Int'l Joint Research] University of Leeds(英国)
• [Int'l Joint Research] Chinese Academy of Sciences(China)
• [Int'l Joint Research] University of Freiburg(Germany)
• [Int'l Joint Research] University of California San Diego(米国)
• [Int'l Joint Research] Shandong University(China)
• [Int'l Joint Research] University of Warwick(英国)
• [Journal Article] Manipulation and Sensing of auxin metabolism, transport and signaling (2018)
  • Author(s): Fukui Kosuke、Hayashi Ken-ichiro
  • Journal Title: Plant and Cell Physiology Volume: 印刷中 Issue: 8 Pages: 1500-1510
  • DOI: 10.1093/pcp/pcy076
  • Peer Reviewed
• [Journal Article] Data-Driven Modeling of Intracellular Auxin Fluxes Indicates a Dominant Role of the ER in Controlling Nuclear Auxin Uptake (2018)
  • Author(s): Middleton Alistair M.、Dal Bosco Cristina、Chlap Phillip、Bensch Robert、Harz Hartmann、Ren Fugang、Bergmann Stefan、Wend Sabrina、Weber Wilfried、Hayashi Ken-ichiro、Zurbriggen Matias D.、Uhl Rainer、Ronneberger Olaf、Palme Klaus、Fleck Christian、Dovzhenko Alexander
  • Journal Title: Cell Reports Volume: 22 Issue: 11 Pages: 3044-3057
  • DOI: 10.1016/j.celrep.2018.02.074
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Feedback from Lateral Organs Controls Shoot Apical Meristem Growth by Modulating Auxin Transport (2018)
  • Author(s): Shi Bihai、Guo Xiaolu、Wang Ying、Xiong Yuanyuan、Wang Jin、Hayashi Ken-ichiro、Lei Jinzhi、Zhang Lei、Jiao Yuling
  • Journal Title: Developmental Cell. Volume: 44 Issue: 2 Pages: 204-216
  • DOI: 10.1016/j.devcel.2017.12.021
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Asymmetric Auxin Distribution is Not Required to Establish Root Phototropism in Arabidopsis (2018)
  • Author(s): Taro Kimura、Ken Haga、Yasushi Shimizu-Mitao、Yumiko Takebayashi、Hiroyuki Kasahara、Ken-ichiro Hayashi、Tatsuo Kakimoto、Tatsuya Sakai
  • Journal Title: Plant & Cell Physiology Volume: 59 Issue: 4 Pages: 828-840
  • DOI: 10.1093/pcp/pcy018
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Yucasin DF, a potent and persistent inhibitor of auxin biosynthesis in plants (2017)
  • Author(s): Tsugafune Shinichi、Mashiguchi Kiyoshi、Fukui Kosuke、Takebayashi Yumiko、Nishimura Takeshi、Sakai Tatsuya、Shimada Yukihisa、Kasahara Hiroyuki、Koshiba Tomokazu、Hayashi Ken-ichiro
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 13992-13992
  • DOI: 10.1038/s41598-017-14332-w
  • Peer Reviewed / Open Access
• [Journal Article] Synergistic action of auxin and cytokinin mediates aluminum‐induced root growth inhibition in Arabidopsis (2017)
  • Author(s): Yang Zhong‐Bao、Liu Guangchao、Liu Jiajia、Zhang Bing、Meng Wenjing、M?ller Bruno、Hayashi Ken‐ichiro、Zhang Xiansheng、Zhao Zhong、De Smet Ive、Ding Zhaojun
  • Journal Title: EMBO reports Volume: 18 Issue: 7 Pages: 1213-1230
  • DOI: 10.15252/embr.201643806
  • Peer Reviewed
• [Journal Article] Aminooxy-naphthylpropionic acid and its derivatives are inhibitors of auxin biosynthesis targeting Trp aminotransferase: Structure-activity relationships (2016)
  • Author(s): M. Narukawa-Nara, A. Nakamura, K. Kikuzato, Y. Kakei, A. Sato, Y. Mitani, Y. Yamasaki-Kokudo, T. Ishii, KI. Hayashi, T. Asami, T. Ogura, S. Yoshida, S. Fujioka, T. Kamakura, T. Kawatsu, M. Tachikawa, K. Soeno, and Y. Shimada,
  • Journal Title: The Plant Journal Volume: 87 Issue: 3 Pages: 245-257
  • DOI: 10.1111/tpj.13197
  • Peer Reviewed / Open Access
• [Journal Article] Development of 4-methoxy-7-nitroindolinyl (MNI)-caged auxins which are extremely stable in planta (2015)
  • Author(s): Hayashi, K., Kusaka, N., Yamasaki, S., Zhao, Y., Nozaki, H.
  • Journal Title: Bioorg Med Chem Lett Volume: 25 Issue: 20 Pages: 4464-4471
  • DOI: 10.1016/j.bmcl.2015.09.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Distinct Characteristics of Indole-3-Acetic Acid and Phenylacetic Acid, Two Common Auxins in Plants. (2015)
  • Author(s): Sugawara, S., Mashiguchi, K., Tanaka, K., Hishiyama, S., Sakai, T., Hanada, K., Kinoshita-Tsujimura, K., Yu, H., Dai, X., Takebayashi, Y., Takeda-Kamiya, N., Kakimoto, T., Kawaide, H., Natsume, M., Estelle, M., Zhao, Y., *Hayashi, K., Kamiya, Y.,Kasahara, H.
  • Journal Title: Plant Cell Physiol Volume: 56 Issue: 8 Pages: 1641-1654
  • DOI: 10.1093/pcp/pcv088
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Distinct characteristics of indole-3-acetic acid and phenylacetic acid, two common auxins in plants (2017)
  • Author(s): S Sugawara, K Mashiguchi, K Tanaka, S Hishiyama, T Sakai, K Hanada, K Kinoshita-Tsujimura, H Yu, X Dai, Y Takebayashi, N Takeda-Kamiya, T Kakimoto, H Kawaide, M Natsume, M Estelle, Y Zhao, K Hayashi, Y Kamiya and H Kasahara
  • Organizer: 植物生理学会 ２０１７年度大会
• [Presentation] 内生植物ホルモン量分析に基づく植物ホルモン受容体制御剤の機能解析研究 (2017)
  • Author(s): 都外川 識志 、宮崎 翔 、尹 禎敏、竹内 純 、轟 泰司 、林 謙一郎 、中嶋 正敏 、浅見 忠 男
  • Organizer: 日本農芸化学会 2017年度大会
• [Presentation] 海産紅藻スサビノリにおけるオーキシンによる先端生長の制御 (2017)
  • Author(s): 田谷賢祐 、林謙一郎 、三上浩司
  • Organizer: 日本植物生理学会２０１７年度大会
• [Presentation] 植物ホルモン・オーキシンのケミカルバイオロジー ホルモン濃度の可視化・自在制御をめざして (2017)
  • Author(s): 林謙一郎
  • Organizer: 東京農工大学グローバルイノベーション研究院・公開セミナー
  • Invited
• [Presentation] HTL/KAI2を活性化する新規物質の合成探索 (2017)
  • Author(s): 福井康祐、浅見忠男、林謙一郎
  • Organizer: 植物化学調節学会 2017年度大会
• [Presentation] Manipulation of cellular auxin distribution by chemical biology approach (2017)
  • Author(s): Ken-ichiro Hayashi, Hiroyasu Motose, Atsushi Funakoshi, Kosuke Fukui, Ryoji Mitsui
  • Organizer: Taiwan-Japan Plant Biology 2017
  • Int'l Joint Research
• [Presentation] 代謝活性化を利用したオーキシン応答の細胞選択的な制御システムの構築 (2016)
  • Author(s): 林謙一郎，船越 淳，本瀬宏康，福井康祐，三井亮司
  • Organizer: 第51回植物化学調節学会
  • Place of Presentation: 高知
  • Year and Date: 2016-10-28
• [Presentation] New auxin analogs as chemical tool for the modulation of cellular PIN localization (2016)
  • Author(s): Ken-ichiro Hayashi
  • Organizer: Auxin2016 conference
  • Place of Presentation: Haitang Bay, Sanya, China
  • Year and Date: 2016-10-20
  • Int'l Joint Research / Invited
• [Presentation] New auxin derivatives that specifically modulate auxin transport via PIN localization (2016)
  • Author(s): Akihiro Oochi, Kosuke Fukui, Hiroyasu Motose, Hiroyuki Kasahara, Richard Napier, Ken-ichiro Hayashi
  • Organizer: Auxin2016 conference
  • Place of Presentation: Haitang Bay, Sanya, China
  • Year and Date: 2016-10-20
  • Int'l Joint Research
• [Presentation] Intracellular auxin gradient is essential for the tip growth of a protonemal cell in the moss, Physcomitrella patens (2016)
  • Author(s): Kousuke Fukui, Akihiro Oochi, Naoki Takeuchi, Hiroyasu Motose, Takashi Aoyama, Tomomichi Fujita, Ken-ichiro Hayashi
  • Organizer: IPGSA2016
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2016-06-21
  • Int'l Joint Research
• [Presentation] Feedback regulation of YUCCA gene expression in Auxin biosynthesis through SCFTIR1/AFB complex (2016)
  • Author(s): Shin Takato, Akiko Sato, Masashi Suzuki, Yusuke Kakei, Ken-ichiro Hayashi, Ayako Nakamura, Kazuo Soeno, Yukihisa Shimada
  • Organizer: IPGSA2016
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2016-06-21
  • Int'l Joint Research
• [Presentation] オーキシン極性輸送はヒメツリガネゴケの原糸体成長を制御する。 (2015)
  • Author(s): 竹内 直樹， 大地 啓寛， 本瀬 宏康， 青山 卓史， 野崎 浩， 藤田 知道， 林 謙一郎
  • Organizer: 第50回植物化学調節学会大会
  • Place of Presentation: 東京大学
  • Year and Date: 2015-10-28
• [Presentation] オーキシン輸送担体PINの局在制御に関するケミカルツール (2015)
  • Author(s): 大地啓寛，本瀬宏康，野崎 浩，林謙一郎
  • Organizer: 第50回植物化学調節学会大会
  • Place of Presentation: 東京大学
  • Year and Date: 2015-10-28