| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In mammals, a master circadian clock is located in the hypothalamic suprachiasmatic nucleus (SCN) which is composed of multiple circadian oscillator neurons and glial cells. Recent studies suggest that the SCN neurons are heterogeneous in not only cytochemical but also oscillatory properties. However, the mechanisms of circadian rhythm stabilization by single neuron cells and glial cells are unknown.
In the present study, I examined circadian properties of a solitary SCN neuron using fluorescent time-lapse imaging and dissociated culture of isolate SCN neurons on spatially isolated microislands. I succeeded to record the circadian intracellular calcium rhythm in a solitary neuron on a microisland. Furthermore, I demonstrated that the circadian calcium rhythm of a solitary neuron coexisting with glial cells on a microisland was disturbed. These results indicate that circadian rhythms in solitary SCN neurons are cell-autonomous, which are destabilized and noise-induced by glial cells.
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