Analysis of neuron-glia interaction related to circadian rhythm generation using the calcium imaging of single suprachiasmatic neuron
| Project/Area Number |
15K01831
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Basic / Social brain science
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 視交叉上核 / 単一神経細胞 / グリア細胞 / カルシウムイメージング / 概日リズム / MEMS / ニューロン / グリア / 光イメージング / 細胞間相互作用 / 生物時計 / 蛍光イメージング / 細胞内カルシウム |
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| Outline of Final Research Achievements |
In mammals, a master circadian clock is located in the hypothalamic suprachiasmatic nucleus (SCN) which is composed of multiple circadian oscillator neurons and glial cells. Recent studies suggest that the SCN neurons are heterogeneous in not only cytochemical but also oscillatory properties. However, the mechanisms of circadian rhythm stabilization by single neuron cells and glial cells are unknown.
In the present study, I examined circadian properties of a solitary SCN neuron using fluorescent time-lapse imaging and dissociated culture of isolate SCN neurons on spatially isolated microislands. I succeeded to record the circadian intracellular calcium rhythm in a solitary neuron on a microisland. Furthermore, I demonstrated that the circadian calcium rhythm of a solitary neuron coexisting with glial cells on a microisland was disturbed. These results indicate that circadian rhythms in solitary SCN neurons are cell-autonomous, which are destabilized and noise-induced by glial cells.
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Report
(4 results)
Research Products
(7 results)
