Analysis of sleep-wake regulatory system by optogenetics featuring cone opsins.
| Project/Area Number |
15K01832
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Basic / Social brain science
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HASEGAWA Emi 筑波大学, 国際統合睡眠医科学研究機構, 助教 (40765955)
SOYA Shingo 筑波大学, 国際統合睡眠医科学研究機構, 助教 (90791442)
SAKURAI Takeshi 筑波大学, 国際統合睡眠医科学研究機構, 教授 (60251055)
MIEDA Michihiro 金沢大学, 医学系, 教授 (20296552)
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| Research Collaborator |
Stefan Herlitze ルール大学, ボーフム・生物学部, 教授
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 睡眠・覚醒 / 光遺伝学 / 錐体オプシン / オレキシン / セロトニン / ノルアドレナリン / ヒスタミン |
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| Outline of Final Research Achievements |
When cone opsins of photoreceptors are artificially expressed in neurons, light irradiation can suppress the neuronal activity and the release of transmitters from the neurons. Applying this method, we analyzed the functional roles of the hypothalamus and the monoaminergic system, which are the core of the sleep-wake regulatory system.
First, reduction of serotonin release in the amygdala has been shown to result in the development of the cataplexy-like episode in the mice model of sleep disorder, narcolepsy. Second, in the fear conditioning test of mice the lateral hypothalamic orexin neurons involved in brain arousal have been elucidated to promote the fear-related behaviors regulated in the amygdala via the activation of noradrenergic neurons.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Serotonin neurons of the dorsal raphe mediate the anti-cataplectic action of orexin neurons by reducing the amygdala activity2017
Author(s)
Hasegawa, E., Maejima, T., Yoshida, T., Masseck O.A., Herlitze, S., Yoshioka, M., Sakurai, T., Mieda, M.
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 114
Issue: 17
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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