chronic measurement of glial dynamics for response to plasticity induction in vivo

• Project/Area Number: 15K01841
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic / Social brain science
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Mishima Tsuneko 国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (90415307)
• Co-Investigator(Renkei-kenkyūsha): HIRASE Hajime 国立研究開発法人理化学研究所, 脳科学総合研究センター, チームリーダー (90392084)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: グリア / 二光子顕微鏡 / 可塑性 / tDCS / ミクログリア / アストロサイト / 免疫組織化学 / カルシウムシグナル / AAV9 / カルシウム

Outline of Final Research Achievements

Transcranial direct current stimulation (tDCS) induce neural and behavioral plasticity. To investigate how this plasticity inducing stimulation affects microglia, which is involved in astrocyte, we performed IBA-1 immunohistochemistry in mouse (C57BL6/J) and IP3R 2 knockout mice brains that underwent tDCS. Morphometric analyses suggest that this microglial cell body enlargement depends on astrocytic Ca2+ activity. Our results parallel with the reduced degree of tDCS-induced astrocytic Ca<SUP>2+</SUP> surges during deep isoflurane or urethane anesthesia and suggest that and their morphological changes may play a role in long-term effects of tDCS. We also perform 2-photone imaging of microglial dynamics before- and after stimulation in vivo, and it suggested that the surveillance area showed the trend to shrink after tDCS. It may the noradrenaline released from neuron by tDCS suppress branch extension of microglia.

Research Products

• [Journal Article] Enhancement of neural plasticity by glial activation : a possible mechanism of transcranial direct current stimulation (2017)
  • Author(s): Hajime Hirase, Tuneko Mishima, Hiromu Monai
  • Journal Title: Dementia Japan Volume: 32 Pages: 17-23
  • NAID: 40021483012
• [Presentation] 経頭蓋直流電気刺激 (tDCS)によるマイクログリア細胞体の変化 (2018)
  • Author(s): 三嶋恒子, 永井てるみ, 矢作和子, 毛内拡, 大江祐樹, 平瀬肇
  • Organizer: 日本神経科学大会（神戸国際会議場）
• [Presentation] Transcranial Direct Current Stimulation Alters Microglial Morphology in Mice (2017)
  • Author(s): Tsuneko Mishima, Yuki Oe, Hiromu Monai, Hajime Hirase
  • Organizer: neuromodulation
  • Place of Presentation: City college of New York、NY
  • Year and Date: 2017-01-12
  • Int'l Joint Research
• [Presentation] Transcranial direct current stimulation alters microglial morphology in mice (2017)
  • Author(s): Tsuneko Mishima, Yuki Oe, Hiromu Monai, Hajime Hirase
  • Organizer: Neuromodulation（New York）
  • Int'l Joint Research
• [Presentation] 経頭蓋直流電気刺激 (tDCS)によるマイクログリア形態変化の解析 (2017)
  • Author(s): 三嶋恒子, 永井てるみ, 矢作和子, 毛内拡, 大江祐樹, 平瀬肇
  • Organizer: 日本神経科学大会(幕張メッセ)