| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
|
|---|
| Outline of Final Research Achievements |
This study described a systematic study of DNA hybridization kinetics on gold nanoparticle (GNPs) with different surface modifications using probe DNA and different molecular weight poly(ethylene glycol)s (PEGs) for detection of Alzheimer's disease-related microRNA (miRNA). A new colorimetric miRNA assay system was developed using DNA/PEG-GNPs and DNA-modified magnetic beads (DNA-MBs). Eventually, target miRNAs could be detected at room temperature without need for other enzymes or instruments, even in the presence of cell lysate and serum. These results show that this system might be a useful tool for usage in Alzheimer's disease diagnosis based on the detection of circulating miRNAs.
|
