| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
In this study, we prepared enzyme-immobilized microbeads via an enzyme cross-linking reaction. The aggregated enzyme on microbeads (PEGA resins) was based on poly-Lys supported cross-linked enzyme aggregates. Compared with the free enzymes, immobilized enzymes were more stable at high temperatures, in the presence of a chemical denaturant, or in an organic solvent. They were recycled without appreciable loss of activity. Immobilized tyrosinase was applied for L-3,4-dihydroxyphenylalanine (L-DOPA) synthesis. L-DOPA is used to treat Parkinson’s disease. Immobilized tyrosinase catalyzed the conversion of tyrosine to L-DOPA. The result was much better than those reported for batch processes that used tyrosinase immobilized on carrier materials. In addition, immobilized laccase was applied for the degradation of endocrine-disrupting chemicals such as bisphenol A. The degradation efficiency of the immobilized laccase was better than those of conventional bioreactors.
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