Development of on-chip cancer diagnostic devices using a high integrated cell chip
| Project/Area Number |
15K04645
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nano/Microsystems
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
yamamura shohei 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ長 (50432141)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 細胞チップ / マイクロアレイ / 循環がん細胞 / 単一細胞解析 / バイオチップ / ハイスループットスクリーニング |
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| Outline of Final Research Achievements |
In this project, we have developed a novel high integrated cell microarray chip system for detection and analysis of circulating tumor cells (CTC). The high integrated cell microarray chip with 78,660 microchambers (100 micrometer diameter) was made from polystyrene by using UV-LIGA process. The chip surface was rendered to hydrophilic by means of reactive-ion etching, which led to the mono-layers formation of leukocytes. Leukocytes isolated from whole blood were dispersed on the chip surface, followed by 15 min standing. Approximately 24 leukocytes (CEM) entered each microchamber when about 1,800,000 leukocytes in total were placed onto the cell microarray chip. In the experiments using spiked breast cancer cell, MCF-7 cells (0.01 to 0.0001%), accurate detection of breast cancer cells was achieved with monoclonal antibodies. Furthermore, we could retrieve target breast cancer cells by light-reactive gas generation polymers or our original micromanipulator system for DNA analysis.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Prognostic Impact of Circulating Tumor Cell Detected Using a Novel Fluidic Cell Microarray Chip System in Patients with Breast Cancer.2016
Author(s)
Sawada T, Araki J, Yamashita T, Masubuchi M, Chiyoda T, Yunokawa M, Hoshi K, Tao S, Yamamura S, Yatsushiro S, Abe K, Kataoka M, Shimoyama T, Maeda Y, Kuroi K, Tamura K, Sawazumi T, Minami H, Suda Y, Koizumi F.
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Journal Title
EBioMedicine.
Volume: 11
Pages: 173-182
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Nanoparticles speckled by ready-to-conjugate lanthanide complexes for multimodal imaging2015
Author(s)
Biju V, Hamada M, Ono K, Sugino S, Ohnishi T, Shibu ES, Yamamura S, Sawada M, Nakanishi S, Shigeri Y, Wakida SI
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Journal Title
Nanoscale
Volume: 5
Issue: 36
Pages: 9511-9516
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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