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Development of component technology for scanning microinjection microscope

Research Project

Project/Area Number 15K04678
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Thin film/Surface and interfacial physical properties
Research InstitutionKogakuin University

Principal Investigator

Takami Tomohide  工学院大学, 教育推進機構(公私立大学の部局等), 教授 (40272455)

Co-Investigator(Renkei-kenkyūsha) IWATA Futoshi  静岡大学, 学術院工学領域, 教授 (30262794)
TAKAKUWA Yuji  東北大学, 多元物質科学研究所, 教授 (20154768)
Research Collaborator SUGAWARA Takeshi  広島大学, 大学院理学研究科, 特任助教 (60713005)
TATE Shin-ichi  広島大学, 大学院理学研究科, 教授 (20216998)
SAITO Mikako  東京農工大学, 大学院工学研究院, 准教授 (20291346)
MATSUOKA Hideaki  東京農工大学, 大学院工学研究院, 名誉教授 (10143653)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsナノピペット / 走査型プローブ顕微鏡 / 細胞工学 / イオン選別 / 走査プローブ顕微鏡 / 触媒 / ナノ粒子 / 回転振動機構 / 真空コンダクタンス / 交流電解研磨 / アクチュエータ / コーティング
Outline of Final Research Achievements

We have developed the following three element technologies to realize scanning microinjection microscope. (1) Motion control during the penetration of the pipette into the cell membrane during injection (2) Development of a non-destructive evaluating method of the cleanliness of the nanopipette inner wall by measuring the entrance pressure dependence of the vacuum conductance through a nanopipette set between two vacuum chambers in order to increase the accuracy of substance injection into cells during injection, and (3) Development of ion-selective nanopipette probe containing ion-selective membrane and establishment of the electrochemical etching of platinum electrode used in scanning pipette microscope.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (16 results)

All 2018 2017 2016 2015

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Open Access: 4 results,  Peer Reviewed: 2 results,  Acknowledgement Compliant: 2 results) Presentation (12 results) (of which Int'l Joint Research: 6 results)

  • [Journal Article] 白金イリジウム線の交流電解研磨における周波数依存性2017

    • Author(s)
      青山宜樹、高見知秀
    • Journal Title

      工学院大学研究報告

      Volume: 123 Pages: 53-58

    • NAID

      40021390040

    • Related Report
      2017 Annual Research Report
    • Open Access
  • [Journal Article] ガラスナノピペット内に作製したセシウムイオン選択膜2017

    • Author(s)
      西山北斗、高見知秀
    • Journal Title

      工学院大学研究報告

      Volume: 123 Pages: 59-64

    • NAID

      40021390048

    • Related Report
      2017 Annual Research Report
    • Open Access
  • [Journal Article] Argon gas flow through glass nanopipette2016

    • Author(s)
      Tomohide Takami, Kiwamu Nishimoto, Tadahiko Goto, Shuichi Ogawa, Futoshi Iwata, and Yuji Takakuwa
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 55 Issue: 12 Pages: 125202-125202

    • DOI

      10.7567/jjap.55.125202

    • NAID

      210000147301

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 細胞への自動インジェクション装置の開発と展望2015

    • Author(s)
      高見知秀
    • Journal Title

      表面科学

      Volume: 36 Issue: 12 Pages: 637-643

    • DOI

      10.1380/jsssj.36.637

    • NAID

      130005115364

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Local ion-selective measurements with nanopipettes and the application2018

    • Author(s)
      TAKAMI, Tomohide,1 WATANABE, Haruka,2 IWATA, Futoshi
    • Organizer
      The 98th Chemical Society of Japan Annual Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 白金イリジウム線の交流電解研磨の周波数依存性2017

    • Author(s)
      高見知秀, 青山宜樹
    • Organizer
      第64回応用物理学会春季学術講演会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2017-03-14
    • Related Report
      2016 Research-status Report
  • [Presentation] Frequency dependence of AC chemical etching of platinum iridium wire2017

    • Author(s)
      Tomohide TAKAMI and Yoshiki AOYAMA
    • Organizer
      33rd Symposium on Chemical Kinetics and Dynamics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of gas flow method for the non-destructive evaluation of glass nanopipette2017

    • Author(s)
      Tomohide TAKAMI, Futoshi IWATA, and Yuji TAKAKUWA
    • Organizer
      254th American Chemical Society National Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ガスフロー法によるガラスナノピペットの非破壊評価法の開発2017

    • Author(s)
      高見知秀, 岩田 太, 高桑雄二
    • Organizer
      第58回真空に関する連合講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] イオン選択ナノピペットの開発2017

    • Author(s)
      高見知秀, 西山北斗, 渡辺 悠
    • Organizer
      第11回分子科学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Vacuum Conductance of Rare Gas through Glass Nanopipette2016

    • Author(s)
      Tomohide Takami, Kiwamu Nishimoto, Tadahiko Goto, Shuichi Ogawa, Futoshi Iwata, Takeshi Sugawara, and Yuji Takakuwa
    • Organizer
      The 20th International Vacuum Congress (IVC-20)
    • Place of Presentation
      BEXCO, Busan
    • Year and Date
      2016-08-21
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 細胞へのインジェクションに用いるナノピペットの非破壊評価法の開発2016

    • Author(s)
      高見知秀,菅原武志
    • Organizer
      生命動態システム科学四拠点合同シンポジウム
    • Place of Presentation
      シェラトンホテル広島
    • Year and Date
      2016-03-25
    • Related Report
      2015 Research-status Report
  • [Presentation] ナノピペットにおける希ガスの真空コンダクタンス~その2~2016

    • Author(s)
      高見知秀, 後藤忠彦, 高桑雄二
    • Organizer
      第63回応用物理学会春季学術講演会
    • Place of Presentation
      東京工業大学大岡山キャンパス
    • Year and Date
      2016-03-19
    • Related Report
      2015 Research-status Report
  • [Presentation] Development of auto-injection system to single cells2015

    • Author(s)
      Tomohide Takami, Mikako Saito, Hideaki Matsuoka, and Shin-ichi Tate
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii Convention Center, Honolulu
    • Year and Date
      2015-12-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Glass Nanopipette Exploring Nanoworld2015

    • Author(s)
      Tomohide Takami
    • Organizer
      The 14th International Symposium on Advanced Technology (ISAT-14)
    • Place of Presentation
      Kogakuin University Hachioji Campus
    • Year and Date
      2015-11-01
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ナノピペットにおける希ガスの真空コンダクタンスの理論計算2015

    • Author(s)
      菅原武志, ○高見知秀,高桑雄二
    • Organizer
      第76回応用物理学会秋季学術講演会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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