Measurement of size, shape, and concentration of particles using confocal dynamic light scattering

• Project/Area Number: 15K04701
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Optical engineering, Photon science
• Research Institution: The Graduate School for the Creation of New Photonics Industries
• Principal Investigator: Ishii Katsuhiro 光産業創成大学院大学, 光産業創成研究科, 准教授 (30311517)
• Project Period (FY): 2015-10-21 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 動的光散乱 / 粒子径計測 / 粒子濃度計測 / 共焦点顕微鏡

Outline of Final Research Achievements

We developed a system of the confocal dynamic light scattering for measurement size, shape, and concentration of particles suspended in water.　A laser beam is introduced to an inverted microscope and is focused on a sample. Scattered light is coupled to a optical-fiber and detected by a photomultiplier tube, and then, the temporal correlation function of scattered light is calculated by a correlator. We showed the developed system can be estimate a size and concentration of particles form results of measurement of polystyrene latex beads. The developed system can be applied to the mixture of two kinds of particles. We also successfully estimated the size and concentration of Bovine serum albumin protein.