• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a new integration method of neutron time-of-flight single-crystal Bragg peaks from a membrane protein crystal

Research Project

Project/Area Number 15K04737
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Quantum beam science
Research InstitutionNational Institutes for Quantum and Radiological Science and Technology (2017)
Japan Atomic Energy Agency (2015)

Principal Investigator

Tomoyori Katsuaki  国立研究開発法人量子科学技術研究開発機構, 高崎量子応用研究所 東海量子ビーム応用研究センター, 研究員(任常) (10455909)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords原子核 / 散乱 / 蛋白質科学 / 生物物理 / 中性子科学 / 量子ビーム / データ処理 / 構造生物 / 熱中性子 / 反射光学 / 数理科学 / 中性子回折 / 原子核物理 / 生体高分子結晶 / 創薬科学 / 酵素反応
Outline of Final Research Achievements

In neutron protein crystallography, the incoherent scattering from hydrogen atoms involved in a protein single-crystal and the surrounding water molecules is significantly large, resulting in backgrounds on neutron diffraction experiment. The diffracted intensities from a protein single-crystal are very weak as the number of reflections increases with larger unit cells. This has difficulty in discriminating between coherent signal and backgrounds especially for the Bragg reflections diffracted from biomacromolecules with large unit-cells. We are planning to build the neutron single-crystal diffractometer in J-PARC/MLF. In this study, I performed the feasibility studies to precisely integrate weak reflections characterized by the single-crystal neutron diffractometry and developed the new data reduction protocol. In addition, I designed a new versatile diffractometer dedicated for biomacromolecules with large unit cells.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2018 2017 2016 2015

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] The design of a versatile TOF neutron diffractometer providing a complementary use of neutron and X-ray diffraction from biomacromolecular single-crystal with large unit cells2018

    • Author(s)
      K. Tomoyori, K. Kurihara, T. Tamada
    • Journal Title

      Journal of Physical Society Japan In press

      Volume: -

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Background elimination using the SNIP algorithm for Bragg reflections from a protein crystal measured by a TOF single-crystal neutron diffractometer2015

    • Author(s)
      K.Tomoyori, Y. Hirano, K. Kurihara, T. Tamada,
    • Journal Title

      Journal of Physics

      Volume: 664 Issue: 7 Pages: 072049-072049

    • DOI

      10.1088/1742-6596/664/7/072049

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] The design of a versatile TOF neutron diffractometer providing a complementary use of neutron and X-ray diffraction from biomacromolecular single-crystal with large unit cells2017

    • Author(s)
      K. Tomoyori, K. Kurihara, T. Tamada
    • Organizer
      International Conference on Neutron Optics (NOP2017), Nara Japan 2017 July
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The design of a TOF neutron diffractometer providing high availability with the combined use of X-ray and neutron scattering from a protein single-crystal with large unit cells.2017

    • Author(s)
      K. Tomoyori, T. Tamada
    • Organizer
      International Conference on Technology and Instrumentation in Particle Physics (TIPP 2017), Beijing China 2017 May
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Data reduction protocol for TOF neutron single protein crystal diffractometry2016

    • Author(s)
      Katsuaki Tomoyori
    • Organizer
      17th International workshop on Advanced Computing and Analysis Techniques in phyiscs research (ACAT2016)
    • Place of Presentation
      UTFSM Valparaiso (Chile)
    • Year and Date
      2016-01-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Background elimination using the SNIP method for Bragg reflections from a protein crystal measured by a time-of-flight single-crystal neutron diffractometer2015

    • Author(s)
      Katsuaki Tomoyori
    • Organizer
      21st International Conference on Computing in High Energy and Nuclear Physics (CHEP 2015)
    • Place of Presentation
      沖縄科学技術大学院大学(OIST) 沖縄県 国頭郡恩納村
    • Year and Date
      2015-04-13
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi