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Non-average structure of particles in liquid studied by fast single-particle analysis using femtosecond X-ray pulses

Research Project

Project/Area Number 15K05407
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Physical chemistry
Research InstitutionJapan Synchrotron Radiation Research Institute

Principal Investigator

Tono Kensuke  公益財団法人高輝度光科学研究センター, XFEL利用研究推進室, 主幹研究員 (00353508)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
KeywordsX線自由電子レーザー / X線構造解析 / X線回折 / 動的構造解析
Outline of Final Research Achievements

Function analysis of biological materials has been performed at the SPring-8 Angstrom Compact Free Electron Laser (SACLA) by using a newly developed experimental system for obtaining dynamical structure of micrometer-scale particles. A pulsed droplet injector was developed as an efficient sample delivery tool in the experimental system, which was successfully applied to the structure analysis of small protein crystals. By combining with an optical laser, this system was able to capture structural dynamics of photo-responsive protein. These studies demonstrate that the new experimental system is highly useful for function analysis of materials.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (18 results)

All 2018 2017 2016 Other

All Int'l Joint Research (4 results) Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Int'l Joint Research: 4 results,  Invited: 3 results) Remarks (1 results)

  • [Int'l Joint Research] Imperial College London/Diamond Light Source(英国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] SLAC National Accelerator Laboratory(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Gothenburg(スウェーデン)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Pohang Univ. of Science and Technology(韓国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Nanosecond pump probe device for time-resolved serial femtosecond crystallography developed at SACLA2017

    • Author(s)
      Kubo Minoru、Nango Eriko、Murata Michio、Neutze Richard、Yabashi Makina、Iwata So, その他18名
    • Journal Title

      Journal of Synchrotron Radiation

      Volume: 24 Issue: 5 Pages: 1086-1091

    • DOI

      10.1107/s160057751701030x

    • NAID

      120006363292

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fluid sample injectors for x-ray free electron laser at SACLA2017

    • Author(s)
      Kensuke Tono
    • Journal Title

      High Power Laser Science and Engineering

      Volume: 5

    • DOI

      10.1017/hpl.2017.6

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature2017

    • Author(s)
      C. Hutchison、V. Cordon-Preciado、R. Morgan、T. Nakane、J. Ferreira、G. Dorlhiac、A. Sanchez-Gonzalez、A. Johnson、A. Fitzpatrick、C. Fare、J. Marangos、C. Yoon、M. Hunter、D. DePonte、S. Boutet、S. Owadai、R. Tanaka、K. Tono、S. Iwata、J. van Thor
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 18 Issue: 9 Pages: 1918-1918

    • DOI

      10.3390/ijms18091918

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 従来の数10分の1の試料で結晶構造解析を可能にする技術を開発2017

    • Author(s)
      真船文隆、武田佳宏、河野淳也、登野健介、宮島謙、宮内直弥
    • Journal Title

      レーザー加工学会誌

      Volume: 24 Pages: 51-54

    • NAID

      40021254980

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SACLAのビームライン・実験装置の概要2017

    • Author(s)
      登野 健介
    • Journal Title

      日本結晶学会誌

      Volume: 59 Pages: 6-11

    • NAID

      130005437773

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Diverse Application Platform for Hard X-ray Diffraction in SACLA (DAPHNIS)2017

    • Author(s)
      Kensuke Tono
    • Journal Title

      Synchrotron Radiation News

      Volume: 30 Issue: 1 Pages: 12-15

    • DOI

      10.1080/08940886.2017.1267555

    • Related Report
      2016 Research-status Report
  • [Journal Article] シリアルフェムト秒結晶構造解析における結晶導入法2016

    • Author(s)
      菅原 道泰、登野 健介、南後 恵理子、岩田 想
    • Journal Title

      放射光

      Volume: 29 Pages: 198-203

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Microcrystals delivery by pulsed liquid droplet for serial femtosecond crystallography2016

    • Author(s)
      Fumitaka Mafune, Ken Miyajima, Kensuke Tono, Yoshihiro Takeda, Jun-ya Kohno, Naoya Miyauchi, Jun Kobayashi, Yasumasa Joti, Eriko Nango, So Iwata, Makina Yabashi
    • Journal Title

      Acta Crystallographica Section D: Biological Crystallography

      Volume: 72 Issue: 4 Pages: 520-523

    • DOI

      10.1107/s2059798316001480

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Recent progress in structural biology at SACLA2018

    • Author(s)
      Kensuke Tono
    • Organizer
      5th Ringberg Meeting on Structural Biology with FELs
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Developments in structural biology at SACLA: Resent results and instrument updates2017

    • Author(s)
      Kensuke Tono
    • Organizer
      4th Ringberg Meeting on Structural Biology with FELs
    • Place of Presentation
      クロイト(ドイツ連邦共和国)
    • Year and Date
      2017-02-08
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Sample delivery methods and instruments at SACLA2016

    • Author(s)
      Kensuke Tono
    • Organizer
      2016 SSRL/LCLS Users’ Meeting
    • Place of Presentation
      メンロパーク(アメリカ合衆国)
    • Year and Date
      2016-10-05
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Platforms for biological researches at SACLA2016

    • Author(s)
      Kensuke Tono
    • Organizer
      12th International Conference on Biology and Synchrotron Radiation
    • Place of Presentation
      メンロパーク(アメリカ合衆国)
    • Year and Date
      2016-08-21
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] XFELと同期したパルス駆動試料インジェクターの開発2016

    • Author(s)
      登野健介、真船文隆、宮島 謙、武田佳宏、河野淳也、宮内直弥、 小林 淳、城地保昌、南後恵理子、岩田 想、矢橋牧名
    • Organizer
      第29回日本放射光学会年会 放射光科学合同シンポジウム
    • Place of Presentation
      柏の葉カンファレンスセンター(千葉県・柏市)
    • Year and Date
      2016-01-09
    • Related Report
      2015 Research-status Report
  • [Remarks] SPring-8 Web Site

    • URL

      http://www.spring8.or.jp/ja/

    • Related Report
      2017 Annual Research Report 2016 Research-status Report 2015 Research-status Report

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Published: 2015-04-16   Modified: 2022-06-14  

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