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Development of multicolor saccharide sensor array and its application for multi-target sensing systems

Research Project

Project/Area Number 15K05526
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Analytical chemistry
Research InstitutionKitami Institute of Technology

Principal Investigator

Kanekiyo Yasumasa  北見工業大学, 工学部, 准教授 (40435748)

Co-Investigator(Kenkyū-buntansha) 青木 寛  国立研究開発法人産業技術総合研究所, エネルギー・環境領域, 主任研究員 (00392580)
Project Period (FY) 2015-10-21 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsボロン酸 / センサ / 過酸化水素 / 次亜塩素酸 / 薄膜 / コンドロイチン硫酸 / アルブミン / グルコース / 糖 / 色素
Outline of Final Research Achievements

Molecular structure of the boronic acid monomer was modified to improve glucose sensitivity. Saccharide sensors were prepared on a pattern-printed microscope slide as a thin film containing newly synthesized boronic acid monomer units. By examining responsiveness against saccharides, it was found that introduction of hydroxylmethyl group onto the aromatic ring of the boronic acid is effective for sensitivity improvement.
The boronic acid-containing thin film was applied for sensing of hydrogen peroxide. By changing monomer composition and dye, variety of color change was realized.
Hypochlorite sensors were also developed. After thoroughly tuning functionality of the thin film, distinct color change appeared.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (37 results)

All 2018 2017 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (33 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Ratiometric Sensing of Hydrogen Peroxide Utilizing Conformational Change in Fluorescent Boronic Acid Polymers2017

    • Author(s)
      Kan Takeshima, Kanako Mizuno, Hitoshi Nakahashi, Hiroshi Aoki, and Yasumasa Kanekiyo
    • Journal Title

      Journal of Analytical Methods in Chemistry

      Volume: 2017 Pages: 7829438-7829438

    • DOI

      10.1155/2017/7829438

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Boronic acid-based thin films that show saccharide-responsive multicolor changes2015

    • Author(s)
      Y. Iwami, T. Yokozawa, H. Yamamoto, Y. Kanekiyo
    • Journal Title

      Journal of Applied polymer Science

      Volume: 132 Issue: 43 Pages: 42679-42679

    • DOI

      10.1002/app.42679

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Thin films exhibiting multicolor changes induced by formaldehyde-responsive release of anionic dyes2015

    • Author(s)
      T. Denda, R. Mizutani, M. Iijima, H. Nakahashi, H. Yamamoto, Y. Kanekiyo
    • Journal Title

      Talanta

      Volume: 144 Pages: 816-822

    • DOI

      10.1016/j.talanta.2015.06.012

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 色調変化型次亜塩素酸センサーの応答特性に及ぼす官能基の影響2018

    • Author(s)
      三谷裕、押田千里、中橋一誌、兼清泰正
    • Organizer
      化学系学協会北海道支部2018年冬季研究発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Colorimetric Polyanion Sensors Utilizing the Dye-Displacement Strategy2018

    • Author(s)
      Hitoshi Nakahashi、Yasumasa Kanekiyo
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Response Characteristics of Novel Colorimetric Sensors for Hypochlorite2018

    • Author(s)
      You Mitani, Yasumasa Kanekiyo
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Novel Strategies for Colorimetric Sensing of Reactive Oxygen Species2018

    • Author(s)
      Yasumasa Kanekiyo
    • Organizer
      9th Edition of International Conference on Analytical Chemistry
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 種々の官能基を導入した薄膜の次亜塩素酸に応答した色調変化挙動2018

    • Author(s)
      兼清泰正、三谷裕
    • Organizer
      第78回分析化学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Novel Colorimetric Sensors for Hypochlorite2017

    • Author(s)
      Chisato Oshida, Hitoshi Nakahashi, Yasumasa Kanekiyo
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶応義塾大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Colorimetric Polyanion Sensors Utilizing Interactions at Thin Film Surface2017

    • Author(s)
      Seong-Eun Pyeon, Chisato Oshida, Yasumasa Kanekiyo
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶応義塾大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Stimuli-Responsive Hydrogels that Exhibit Responsiveness against Various Molecules2017

    • Author(s)
      Masato Hosoda, Yasumasa Kanekiyo
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶応義塾大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 新規応答メカニズムに基づく色調変化型次亜塩素酸センサーの作製2017

    • Author(s)
      押田千里・中橋一誌・兼清泰正
    • Organizer
      化学系学協会北海道支部2017年冬季研究発表会
    • Place of Presentation
      北海道大学
    • Year and Date
      2017-01-17
    • Related Report
      2016 Research-status Report
  • [Presentation] ボロン酸ポリマーを用いた活性酸素種の蛍光センシング2017

    • Author(s)
      水野佳那子・竹島完・兼清泰正
    • Organizer
      化学系学協会北海道支部2017年冬季研究発表会
    • Place of Presentation
      北海道大学
    • Year and Date
      2017-01-17
    • Related Report
      2016 Research-status Report
  • [Presentation] 様々な刺激に応答するヒドロゲルの設計と特性解析2017

    • Author(s)
      細田誠人・兼清泰正
    • Organizer
      化学系学協会北海道支部2017年冬季研究発表会
    • Place of Presentation
      北海道大学
    • Year and Date
      2017-01-17
    • Related Report
      2016 Research-status Report
  • [Presentation] ポリアニオンに応答して明瞭な色調変化を示す薄膜型センサーの創製2017

    • Author(s)
      Pyeon, Seong Eun、兼清泰正、押田千里
    • Organizer
      第77回分析化学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 刺激応答性アミロースのヨウ素包摂錯体形成機能を利用した新規センシング法2017

    • Author(s)
      兼清泰正、土屋政元
    • Organizer
      第17回リング・チューブ超分子研究会シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 新規メカニズムに基づく色調変化型次亜塩素酸センサーの応答特性2017

    • Author(s)
      三谷裕、押田千里、中橋一誌、兼清泰正
    • Organizer
      日本化学会北海道支部2017年夏季研究発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ボロン酸含有薄膜と酵素との組み合わせによる高感度高選択的色調変化型センサーの開発2017

    • Author(s)
      中橋一誌、三谷裕、兼清泰正
    • Organizer
      日本化学会北海道支部2017年夏季研究発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ボロン酸含有薄膜を用いた色調変化型糖センサーの酵素機能による高感度化2017

    • Author(s)
      中橋一誌、三谷裕、兼清泰正
    • Organizer
      第11回バイオ関連化学シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 多様な分子刺激に応答するヒドロゲルの設計と特性解析2017

    • Author(s)
      細田誠人、藤井剛、兼清泰正
    • Organizer
      第66回高分子討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 新規メカニズムに基づく次亜塩素酸応答性薄膜の色調変化挙動2017

    • Author(s)
      三谷裕、押田千里、中橋一誌、兼清泰正
    • Organizer
      日本分析化学会第66年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 生体高分子に応答して明瞭多彩な色調変化を示す薄膜型センサーの創製2017

    • Author(s)
      中橋一誌、Pyeon, Seong Eun、兼清泰正
    • Organizer
      日本分析化学会第67年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 刺激応答性アミロースを用いたヨウ素-デンプン反応による新規センシング法2017

    • Author(s)
      兼清泰正、土屋政元
    • Organizer
      2017ハロゲン利用ミニシンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 様々な分子に対して膨潤・収縮応答を示すヒドロゲルの開発2016

    • Author(s)
      細田 誠人 ・ 兼清 泰正
    • Organizer
      第6回CSJ化学フェスタ2016
    • Place of Presentation
      タワーホール船堀
    • Year and Date
      2016-11-14
    • Related Report
      2016 Research-status Report
  • [Presentation] 様々な分子を認識して多彩な色調変化を示す薄膜型センサーの創製2016

    • Author(s)
      兼清泰正
    • Organizer
      日本分析化学会第65年会
    • Place of Presentation
      北海道大学
    • Year and Date
      2016-09-14
    • Related Report
      2016 Research-status Report
  • [Presentation] 蛍光基含有ボロン酸ポリマーの分子認識機能に及ぼすボロン酸構造の影響2016

    • Author(s)
      竹島 完 ・ 水野 佳那子 ・ 兼清 泰正
    • Organizer
      日本分析化学会第65年会
    • Place of Presentation
      北海道大学
    • Year and Date
      2016-09-14
    • Related Report
      2016 Research-status Report
  • [Presentation] 新規応答メカニズムに基づく色調変化型の次亜塩素酸センサー作製2016

    • Author(s)
      中橋 一誌 ・ 押田 千里 ・ 兼清 泰正
    • Organizer
      日本分析化学会第65年会
    • Place of Presentation
      北海道大学
    • Year and Date
      2016-09-14
    • Related Report
      2016 Research-status Report
  • [Presentation] Dye-Displacement Strategies for Colorimetric Sensing against Various Compounds2016

    • Author(s)
      Yasumasa Kanekiyo
    • Organizer
      ISAMR2016
    • Place of Presentation
      台湾・日月潭
    • Year and Date
      2016-08-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular Recognition of Reactive Oxygen Species and Nucleosides by Fluorescent Boronic Acid Polymers2016

    • Author(s)
      Kan TAKESHIMA, Yasumasa KANEKIYO
    • Organizer
      ISAMR2016
    • Place of Presentation
      台湾・日月潭
    • Year and Date
      2016-08-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Highly Sensitive Glucose Sensors Created by Combining Boronic Acid-Containing Thin Films with Enzymes2016

    • Author(s)
      Hitoshi NAKAHASHI, Yasumasa KANEKIYO
    • Organizer
      ISAMR2016
    • Place of Presentation
      台湾・日月潭
    • Year and Date
      2016-08-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ボロン酸含有薄膜と酵素の組み合わせによる高感度グルコースセンサーの開発2016

    • Author(s)
      兼清 泰正、中橋 一誌
    • Organizer
      第76回分析化学討論会
    • Place of Presentation
      岐阜薬科大学・岐阜大学
    • Year and Date
      2016-05-28
    • Related Report
      2016 Research-status Report 2015 Research-status Report
  • [Presentation] Novel Hydrogels That Exhibit Responsiveness against Various Molecules2016

    • Author(s)
      Tsuyoshi Fujii, Yasumasa Kanekiyo
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学
    • Year and Date
      2016-03-24
    • Related Report
      2015 Research-status Report
  • [Presentation] Molecular Recognition by Fluorescent Boronic Acid Polymers2016

    • Author(s)
      Kan Takeshima, Yasumasa Kanekiyo
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学
    • Year and Date
      2016-03-24
    • Related Report
      2015 Research-status Report
  • [Presentation] Highly Sensitive Glucose Sensors Created by Combining Boronic Acid-Containing Thin Films and Enzymes2016

    • Author(s)
      Hitoshi Nakahashi, Yasumasa Kanekiyo
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学
    • Year and Date
      2016-03-24
    • Related Report
      2015 Research-status Report
  • [Presentation] 蛍光基含有ボロン酸ポリマーが示す多様な分子認識機能2016

    • Author(s)
      竹島 完・兼清 泰正
    • Organizer
      日本化学会北海道支部2016年夏季研究発表会
    • Place of Presentation
      室蘭工業大学
    • Related Report
      2016 Research-status Report
  • [Presentation] 新たな分子応答性ヒドロゲルの設計と応答特性解析2016

    • Author(s)
      細田 誠人・兼清 泰正
    • Organizer
      日本化学会北海道支部2016年夏季研究発表会
    • Place of Presentation
      室蘭工業大学
    • Related Report
      2016 Research-status Report
  • [Book] Boron: Sensing, Synthesis and Supramolecular Self-Assembly2015

    • Author(s)
      Y. Kanekiyo, S. Shinkai
    • Total Pages
      416
    • Publisher
      RSC Publishing
    • Related Report
      2015 Research-status Report

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Published: 2015-10-21   Modified: 2019-03-29  

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