Imaging of alkali ion dynamics in cells using protein-coupled fluorescence probes
| Project/Area Number |
15K05529
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Saitama University (2016-2017)
The University of Tokyo (2015) |
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Principal Investigator |
Terai Takuya 埼玉大学, 理工学研究科, 特別研究員 (00508145)
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| Research Collaborator |
Nemoto Naoto 埼玉大学, 大学院理工学研究科, 教授 (60509727)
Anzai Hiroki 埼玉大学, 大学院理工学研究科, 大学院生
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 分子認識 / 合成化学 / 共焦点顕微鏡 / 蛍光センサー |
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| Outline of Final Research Achievements |
Alkali metals (sodium, potassium) play important physiological functions in organisms, but their spatiotemporal dynamics is not well understood. In this research, we developed new organic fluorescence probes for these cations, and by conjugation of the probes to tag proteins that were expressed in some organelles, we succeeded in fluorescence imaging of target ions at the organelles of interest in live cells. Because this method has generality, it will be possible to create practical imaging tools for understanding other ion dynamics in cells.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Synthesis of Practical Red Fluorescent Probe for Cytoplasmic Calcium Ions with Greatly Improved Cell-membrane Permeability2017
Author(s)
Kazuhisa Hirabayashi, Kenjiro Hanaoka, Takahiro Egawa, Chiaki Kobayashi, Shodai Takahashi, Toru Komatsu, Tasuku Ueno, Takuya Terai, Yuji Ikegaya, Tetsuo Nagano, Yasuteru Urano
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Journal Title
Data in Brief
Volume: 12
Pages: 351-357
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Discovery of Cell-type-specific and Disease-related Enzymatic Activity Changes via Global Evaluation of Peptide Metabolism2017
Author(s)
Jun Onagi, Toru Komatsu, Yuki Ichihashi, Yugo Kuriki, Mako Kamiya, Takuya Terai, Tasuku Ueno, Kenjiro Hanaoka, Hiroyuki Matsuzaki, Keisuke Hata, Toshiaki Watanabe, Tetsuo Nagano, and Yasuteru Urano
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Journal Title
J. Am. Chem. Soc.
Volume: 139
Issue: 9
Pages: 3465-3472
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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