| Project/Area Number |
15K05530
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
GODA Tatsuro 東京医科歯科大学, 生体材料工学研究所, 助教 (20588347)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | バイオトランジスタ / 翻訳後修飾 / ヒストン / アセチル化 / 水晶発振振動子 / 表面プラズモン共鳴 / DNAアプタマー / タンパク質吸着 / ＤＮＡアプタマー / 電荷密度 / 核酸アプタマー |
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| Outline of Final Research Achievements |
We developed a method to electrically detect the post-translational modification of proteins, a key biological phenomena, using "bio-transistor" which directly detects the charge density change of biomolecules at the electrode/solution interface. This method is label free, without using fluorescent dyes, thereby realizing reduction of analysis time and cost, miniaturization of the apparatus, high throughput, and point-of-care diagnosis of the post-translational modification. Our achievements lead to the provision of bio-transistor as a unified platform that evaluates multi-modal biological information ranged from genome to epigenome, tanscriptome, and post-translational modification. It is a challenging research subject directly linked to the next-generation medical treatment advocating decentralized ubiquitous medical infrastructures using portable devices while providing high-quality diagnosis by overcoming false positive/false negative problems through comprehensive analysis.
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