Development of a near-field tomographic imaging technique for SPR and fluorescence by using shorter wavelength light

• Project/Area Number: 15K05535
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: University of Toyama
• Principal Investigator: SUZUKI MASAYASU 富山大学, 大学院理工学研究部(工学), 教授 (70226554)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: バイオセンサ / 表面プラズモン共鳴センサ / 近接場イメージング / 計測工学 / 細胞イメージング

Outline of Final Research Achievements

Significant advances are shown in imaging techniques for cell membrane surface and cellular cytoplasm, but few studies on local precise imaging techniques for juxtamembrane region have been reported. This study focused on realization for the simultaneous imaging of surface plasmon resonance (SPR) and surface plason enhanced fluorescence (SPEF). Novel sensor chips were developed for SPR imaging under shorter wavelength light. Simultaneous imaging of SPR and SPEF could be realized by using these sensor chips and shorter wavelength light.

Research Products

• [Journal Article] 単一細胞解析のためのバイオセンシング技術 (2017)
  • Author(s): 鈴木正康
  • Journal Title: 化学工業 Volume: 68 Pages: 96-100
• [Journal Article] Microscale chemical and enzyme sensor arrays prepared by microcontact printing (2015)
  • Author(s): M.Suzuki, A.Nomura, M.Yamamoto, K.Minakuchi, Y.Iribe
  • Journal Title: Sensors and Materials Volume: 27 Pages: 881-889
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Al-Al2O3薄膜を用いたＳＰＲーＳＰＥＦ同時計測 (2017)
  • Author(s): 堂浜光司、入部康敬、鈴木正康
  • Organizer: 2017年電気化学会北陸支部秋季大会
• [Presentation] 酵素ーポリアニリン複合体の微小化とＳＰＲによる測定 (2017)
  • Author(s): 礒野幸平、小林大和、東野志保、入部康敬、鈴木正康
  • Organizer: 2017年電気化学会北陸支部秋季大会
• [Presentation] Enzyme switches on SPR imaging sensor chip (2016)
  • Author(s): M.Suzuki, Y.Kobayashi, I.Murai, Y.Iribe
  • Organizer: 26th Anniversary World Congress on Biosensors
  • Place of Presentation: The Swedish Exhibition and Cogress Centre, Goteborg, Sweden
  • Year and Date: 2016-05-24
  • Int'l Joint Research
• [Presentation] Low-cost biosensor chips for BOD and pesticides monitoring (2016)
  • Author(s): M.A.Kashem,K.Kimoto, Y.Iribe, M.Suzuki
  • Organizer: 26th Anniversary World Congress on Biosensors
  • Place of Presentation: The Swedish Exhibition and Cogress Centre, Goteborg, Sweden
  • Year and Date: 2016-05-24
  • Int'l Joint Research
• [Presentation] ＳＰＲ－ＳＰＥＦ同時計測のためのＳＰＲセンサ膜の検討 (2016)
  • Author(s): 堂浜光司, 入部康敬, 鈴木正康
  • Organizer: 2016年電気化学会北陸支部秋季大会
  • Place of Presentation: 富山大学工学部, 富山
• [Presentation] 酸素計測蛍光膜を用いた簡易なBODセンサチップの開発 (2016)
  • Author(s): M.A.Kashem, A.B.A.Tharmizi, 入部康敬, 鈴木正康
  • Organizer: 2016年電気化学会北陸支部秋季大会
  • Place of Presentation: 富山大学工学部, 富山
• [Presentation] 短波長光源を利用した２Ｄ－ＳＰＲイメージングの実現とその応用 (2015)
  • Author(s): 入部康敬、鈴木正康
  • Organizer: 化学とマイクロ・ナノシステム学会第３２回研究会
  • Place of Presentation: 北九州国際会議場
  • Year and Date: 2015-11-26