| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Bacteriophages are viruses that infect only bacterial cells in order to reproduce. Their natural affinity to the host cells can be used to design highly specific tools. We proposed phage-conjugated silica-gold core-shell nanoparticles as plasmon scattering probes for dark-field optical detection of pathogenic bacteria. By electrostatic layer-by-layer deposition on the silica nanoparticles, the phage-conjugated silica-gold core-shell nanoparticles were prepared. The phage-conjugated silica-gold core-shell nanoparticles selectively bound to the surfaces of target bacteria. The nanoparticles-bound bacteria remarkably increased the light scattering intensity. Our result suggests that the pathogenic bacteria can be detected with high selectivity and sensitivity by the dark-filed microscopy technique. Furthermore, a wide variety of bacteria can be rapidly detected by altering a variety of phages immobilized on the nanoparticles.
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