Development and application of phage interfaces for bacteria detection

• Project/Area Number: 15K05541
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kochi University
• Principal Investigator: Watanabe Shigeru 高知大学, 教育研究部総合科学系複合領域科学部門, 教授 (70253333)
• Co-Investigator(Kenkyū-buntansha): 波多野 慎悟 高知大学, 教育研究部総合科学系複合領域科学部門, 講師 (70397157)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 金ナノ粒子 / ファージ / 細菌 / 暗視野顕微鏡 / 比色検出 / 光散乱 / バクテリオファージ / 凝集 / 静電相互作用

Outline of Final Research Achievements

Bacteriophages are viruses that infect only bacterial cells in order to reproduce. Their natural affinity to the host cells can be used to design highly specific tools. We proposed phage-conjugated silica-gold core-shell nanoparticles as plasmon scattering probes for dark-field optical detection of pathogenic bacteria. By electrostatic layer-by-layer deposition on the silica nanoparticles, the phage-conjugated silica-gold core-shell nanoparticles were prepared. The phage-conjugated silica-gold core-shell nanoparticles selectively bound to the surfaces of target bacteria. The nanoparticles-bound bacteria remarkably increased the light scattering intensity. Our result suggests that the pathogenic bacteria can be detected with high selectivity and sensitivity by the dark-filed microscopy technique. Furthermore, a wide variety of bacteria can be rapidly detected by altering a variety of phages immobilized on the nanoparticles.

Research Products

• [Journal Article] Rapid and Sensitive Detection of Protein Glycosylation Based on Lectin-induced Aggregation of Lactoferrin-modified Gold Nanoparticles (2016)
  • Author(s): 東 優磨, 清岡千尋, 塚本英視, 波多野慎悟, 渡辺 茂
  • Journal Title: Milk Science Volume: 65 Issue: 2 Pages: 71-80
  • DOI: 10.11465/milk.65.71
  • NAID: 130005263653
  • ISSN: 1343-0289, 2188-0700
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] バクテリオファージ担持金ナノ粒子凝集体を利用した細菌の暗視野顕微鏡検出 (2018)
  • Author(s): 今井 斉志，鷲尾 和也，仁子 陽輔，波多野 慎悟，渡辺 茂，松崎 茂展，内山 淳平
  • Organizer: 日本化学会第98春季年会
• [Presentation] 細菌検出に向けたカチオン性金ナノ粒子の開発 (2017)
  • Author(s): 鷲尾和也，石澤 駿，仁子陽輔，波多野 慎悟，渡辺 茂
  • Organizer: 日本化学会 第97春季年会 (2017)
  • Place of Presentation: 慶應義塾大学 日吉キャンパス（神奈川県・横浜市）
  • Year and Date: 2017-03-16
• [Presentation] ベイエリア環化型ペリレンビスイミドの合成とその光物理的性質 (2017)
  • Author(s): 関田慎也，古賀智子，仁子陽輔，波多野 慎悟，渡辺 茂
  • Organizer: 日本化学会 第97春季年会 (2017)
  • Place of Presentation: 慶應義塾大学 日吉キャンパス（神奈川県・横浜市）
  • Year and Date: 2017-03-16
• [Presentation] バクテリオファージ担持ナノ粒子の調製と細菌検出への応用 (2017)
  • Author(s): 今井斉志，鷲尾和也，仁子陽輔，波多野 慎悟，渡辺 茂，松崎茂展，内山順平
  • Organizer: 日本食品微生物学会第38回学術総会
• [Presentation] 金ナノ粒子を利用した細菌の比色検出に関する基礎研究 (2015)
  • Author(s): 塚本英視, 波多野慎悟, 渡辺 茂
  • Organizer: 第38回溶液化学シンポジウム
  • Place of Presentation: 高知市文化プラザかるぽーと（高知県・高知市）
  • Year and Date: 2015-10-21