Design and Synthesis of Photo-cleavable Macromolecular Tag for LDI-MS

• Project/Area Number: 15K05543
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Nagasaki University
• Principal Investigator: MAKI Toshihide 長崎大学, 産学官連携戦略本部, 准教授 (10291535)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ＬＩＤ-ＭＳ / 質量分析 / 分子タグ / ポルフィリン / 分子対称性 / アミン / LDI-MS / 誘導化反応 / 表面反応 / ヒドロキシスクシンイミドエステル / 標識剤 / イオン化

Outline of Final Research Achievements

This research was aiming for establish of new molecular tag for LDI-MS which is not sensitive for molecular weight of target molecules. Tetraphenylporphyrine was found as a good motif to afford efficiently stable and detectable ion for LDI-MS. The symmetrical molecule reacted with sample amines in solution phase afforded statistical product distribution on mass spectrometry. That is reactivity of molecule or yield was able to read out form its mass spectra. When the organic film formed by the molecular tag lowered detection limit less than tens pico-mol for each film spotted. The result suggests the system can be used for assessment for surface reactivity and the system has a chance minimize diffusion area which is believed to lead lower resolution in imaging mass spectrometry.