| Project/Area Number |
15K05546
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ISHIHAMA Yasushi 京都大学, 薬学研究科, 教授 (30439244)
MURAKAMI Hiroya 愛知工業大学, 工学部, 准教授 (40515128)
TORIMURA Masaki 国立研究開発法人産業技術総合研究所, イノベーション推進本部, 総括企画主幹 (40357588)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | CEオンライン濃縮 / オンライン錯体化 / 発がんリスクマーカー / 損傷ヌクレオチド / ESI-MS / Bal31 / D化内部標準 / 自動プログラム / CEオンライン濃縮注入 / 損傷DNA / ナノESI-MS / ESI効率 / ナノＥＳＩ－ＭＳ |
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| Outline of Final Research Achievements |
We have established a basis for highly sensitive detection of DNA damages occurred by drinking, developing capillary electorophoresis-mass spectrometry methods. The damaged bases were extracted from DNA as the corresponding 5’-nucleotides, and were enriched 3000-times by electrokinetic supercharging followed by on-line complexation with the Phostag reagent to enhance ESI efficiency of the nucleotides and finally were introduced to an ESI-MS system. The sensitivity would be more than 30,000-times higher compared with that with detections as the nucleotides by using a simple CE-ESI-MS method.
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