Development of ultra sensitive CE-ESI-MS methods based on the nano-focusng stratedy
Project/Area Number |
15K05546
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Analytical chemistry
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Research Institution | Gifu Pharmaceutical University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
ISHIHAMA Yasushi 京都大学, 薬学研究科, 教授 (30439244)
MURAKAMI Hiroya 愛知工業大学, 工学部, 准教授 (40515128)
TORIMURA Masaki 国立研究開発法人産業技術総合研究所, イノベーション推進本部, 総括企画主幹 (40357588)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
|
Keywords | CEオンライン濃縮 / オンライン錯体化 / 発がんリスクマーカー / 損傷ヌクレオチド / ESI-MS / Bal31 / D化内部標準 / 自動プログラム / CEオンライン濃縮注入 / 損傷DNA / ナノESI-MS / ESI効率 / ナノESI-MS |
Outline of Final Research Achievements |
We have established a basis for highly sensitive detection of DNA damages occurred by drinking, developing capillary electorophoresis-mass spectrometry methods. The damaged bases were extracted from DNA as the corresponding 5’-nucleotides, and were enriched 3000-times by electrokinetic supercharging followed by on-line complexation with the Phostag reagent to enhance ESI efficiency of the nucleotides and finally were introduced to an ESI-MS system. The sensitivity would be more than 30,000-times higher compared with that with detections as the nucleotides by using a simple CE-ESI-MS method.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Endogenous Adenosine May Be Related to Left Ventricular Dysfunction, Dilation, and Wall Thinning in Patients With Heart Disease2018
Author(s)
Y. Kinomura, T. Tanaka, T. Aoyama ,M. Hatasa, S. Minatoguchi, M. Iwasa, Y. Yamada, T. Nawa, H. Kanamori, M. Kawasaki, Y. Esaka, B. Uno, K. Nishigaki, S. Minatoguchi
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Journal Title
Circulation Journal
Volume: 82
Issue: 5
Pages: 1319-1326
DOI
NAID
ISSN
1346-9843, 1347-4820
Related Report
Peer Reviewed
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