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Preparation of Ti-binding osteogenic protein by using phosphorylated chitin

Research Project

Project/Area Number 15K05554
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bio-related chemistry
Research InstitutionHokkaido University

Principal Investigator

KUBOKI Yoshinori  北海道大学, 地球環境科学研究院, 名誉教授 (00014001)

Co-Investigator(Kenkyū-buntansha) 田中 俊逸  北海道大学, 地球環境科学研究院, 特任教授 (30142194)
戸倉 清一  北海道大学, 地球環境科学研究院, 名誉教授 (40000806)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsリン蛋白質 / チタン / 人工骨 / 人工歯根 / キチン / リン酸化キチン / チタン結合性蛋白質 / チタンと骨の結合 / 3次元マトリックス / チタン結合性骨造成蛋白質 / キチンのリン酸化 / リン酸化キチンのチタン結合性 / 骨中のリン蛋白 / インプラント蛋白質 / 骨とチタンの結合メカニズム / リン酸化セリン / チタン結合性蛋白 / チタン骨内定着 / 骨シアロ蛋白 / RGD細胞接着配列 / 人工関節 / 骨形成 / インプラント / 合成蛋白
Outline of Final Research Achievements

In 2014, we discovered that bone phosphoproteins, which are collectively called SIBLING proteins family, possessed the titanium-binding ability. Furthermore, the titanium implant devices which were coated with these titanium binding proteins (TiBP) induced more than 100 times higher amount of bone than the non-coated control. Inspired by this discovery, we chemically phosphorylated chitin to see whether the same effect can occur in the phosphorylated chitin (P-chitin). The result was that P-chitin definitely bound with titanium, and P-chitin coated titanium implant induced several times higher amount of bone, when implanted into rat calvaria. We attributed the unique property of P-chitin to the regular arrangement of phosphorylated groups of N-acetyl glucosamine corresponding with regular crystal units of TiO2. Usefulness of the new findings are self-evident for dental and orthopedic implants.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (33 results)

All 2018 2017 2016 2015

All Journal Article (11 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 11 results,  Acknowledgement Compliant: 1 results,  Open Access: 1 results) Presentation (20 results) (of which Int'l Joint Research: 5 results,  Invited: 7 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] New principles of regenerative medicine: special reference to mechano-dynamic factors2017

    • Author(s)
      Yoshnori Kuboki, Kimitoshi Yagami and Toshitake Furusawa
    • Journal Title

      J. Oral Tissue Engineering

      Volume: 14 Pages: 128-152

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical Mechanism of Titanium Fixation into Living Bone: Acid Soluble Phosphoproteins in Bone Binds with Titanium and Induced Endochondral Ossification in vivo2017

    • Author(s)
      Yoshinori KUBOKI, Kimitoshi YAGAMI, Michiko TERADA-NAKAISHI, Toshitake FURUSAWA, Yasuko NAKAOKI, and Masaaki KURASAKI
    • Journal Title

      J. Oral Tissue Engineering

      Volume: 15 Pages: 109-118

    • NAID

      130006394591

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adsorption of copper and zinc onto carbon material in an aqueous solution oxidized by ammonium peroxydisulphat2017

    • Author(s)
      Y.Dai, K.Zhang,J.Li, Y.Jiang, Y.Chen and S.Tanaka
    • Journal Title

      Separation and Purification Technology

      Volume: 186 Pages: 255-263

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical synthesis and immobilization of a beadwork-like Prussian Blue on carbon fiber and the removal of cesium e2017

    • Author(s)
      A.Yamashita and S.Tanaka
    • Journal Title

      Journal of Environmental Chemical Engineering

      Volume: 5 Pages: 2912-2920

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of anti-gravity apparatus for studying gravity effects on cell culture2016

    • Author(s)
      Yoshinori Kuboki, Kimitoshi Yagami, Seiichi Tokura, Masaaki Kurasaki, Shingo Ogawa, Hiroko Takita, Rachel Sammons:
    • Journal Title

      J. Oromaxillofacial Biomechanics

      Volume: 21 Pages: 9-17

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 再生医療におけるナノファイバー応用の原理:人工細胞外マトリックスの幾何学2016

    • Author(s)
      久保木芳徳
    • Journal Title

      Nanofiber

      Volume: 7 Pages: 26-34

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] 1)Study on the mechanism of lead adsorption using nitric treated carbon material2016

    • Author(s)
      Yingjie Dai1, Changjie Du, Haijiao Yu, Danfeng Zhang, Shunitz Tanaka
    • Journal Title

      Fresenius Environmental Bulletin

      Volume: 25 Pages: 3518-3522

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] 2)Evaluation of antimicrobial activities of some traditional medicinal plants of Bangladesh2016

    • Author(s)
      Parvin Begum and Shunitz Tanaka
    • Journal Title

      Journal of Science and Research Methodology

      Volume: 2 Pages: 1-16

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] 3)Shirasu Balloons and Polydopamine-Modified Shirasu Balloons for Adsorption of Methylene Blue2016

    • Author(s)
      Chairunnisa, Dwi Siswanta, Adhitasari Suratman and ShunitzTanaka
    • Journal Title

      Water Air Soil Pollution

      Volume: 227 Pages: 412-412

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] 4)Adsorption kinetic model of alginate gel beads synthesized micro particle-Prussian Blue to remove cesium ions from water2016

    • Author(s)
      Mihara, Y., Sikder, M.T., Yamagishi, H., Sasaki. T., Kurasaki. M., Itoh. S., Tanaka. S.,
    • Journal Title

      Journal of Water Process Engineering

      Volume: 10 Pages: 9-19

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Atelocollagen Enhanced Osteogenesis in a Geometric Structured Beta-TCP Scaffold by VEGF Induction,2016

    • Author(s)
      Kimitoshi Yagami, Sunao Sadaoka, Hiroshi Nakamura, Saho Komatsu, Jun Onodera, Masahiko Suzuki and iYoshinori Kuboki
    • Journal Title

      J Tissue Sci Eng

      Volume: 7 Issue: 01 Pages: 1-8

    • DOI

      10.4172/2157-7552.1000162

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 反重力培養装置はDFAT細胞の増殖を促進する2018

    • Author(s)
      久保木芳徳,Parvin Begum, 藏崎正明、戸倉清一, 松本太郎
    • Organizer
      第30回代用臓器・再生医学研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] バングラデッシュ飲料水のヒ素除染を目指した新型多孔性粒状アパタイト体の開発2018

    • Author(s)
      久保木芳徳, Parvin Begum,Myeenuddin Chowdhury, 戸倉清一, 藏崎正明:
    • Organizer
      第30回代用臓器・再生医学研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] キチンとキトサンはキチンと分けて考えよう、シンポジウム:次世代のマトリックスは再生医療の新しい扉を開く2017

    • Author(s)
      戸倉清一、久保木芳徳
    • Organizer
      第16回日本再生医療学会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2017-03-09
    • Related Report
      2016 Research-status Report
  • [Presentation] リン酸化キチンは、チタンに結合し骨を誘導する, シンポジウム:次世代のマトリックスは再生医療の新しい扉を開く2017

    • Author(s)
      久保木芳徳, 八上公利, 古澤利武,Parvin Begum,Mostafizur Rahman 藏崎正明, 戸倉清一
    • Organizer
      第16回日本再生医療学会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2017-03-09
    • Related Report
      2016 Research-status Report
  • [Presentation] 生体高分子の化学リン酸化の意義:臓器再建と環境浄化の基礎素材としての機能2017

    • Author(s)
      久保木芳徳,戸倉清一, 藏崎正明、Parvin Begum、坂入信夫
    • Organizer
      第29回代用臓器・再生医学研究会
    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Presentation] 生体高分子の化学リン酸化によるチタン結合性の獲得(Acquisition of titanium-biding ability by chemical phosphorylation of bio-molecules)2017

    • Author(s)
      久保木芳徳、八上公利、古沢利武、戸倉清一
    • Organizer
      第59回歯科基礎医学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Phosphorylation of the biomolecules enabled to acquire titanium-binding and bone-enhancing ability when coated on the surface of titanium implants2017

    • Author(s)
      Y. Kuboki, S. Tokura, T. Furusawa, K. Yagami
    • Organizer
      The 6th International Conference of Biomedical Engineering and Biotechnology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Identification of Hydroxyapatite-Interacting Proteins in Bone2017

    • Author(s)
      Hai-Yan Zhou, Yoshinori Kuboki
    • Organizer
      The 6th International Conference of Biomedical Engineering and Biotechnology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Principle of Regenerative Medicine: Geometry of the Artificial Extracellular Matrix2016

    • Author(s)
      Yoshinori Kuboki
    • Organizer
      Conference of regenerative medicine and environmental purification
    • Place of Presentation
      海口市(中国)
    • Year and Date
      2016-11-18
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] New concepts for regenerative medicine and environmental purification2016

    • Author(s)
      Yoshinori Kuboki
    • Organizer
      Conference of regenerative medicine and environmental purification
    • Place of Presentation
      海口市(中国)
    • Year and Date
      2016-11-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] チタンの骨内定着の生化学メカニズム解明・インプラント蛋白の意義と応用2016

    • Author(s)
      久保木芳徳, 八上公利, 古澤利武
    • Organizer
      シンポジウム 21世紀の歯科インプラントは基礎と臨床の融合から、第58回歯科基礎医学会学術大会
    • Place of Presentation
      札幌市コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-08-24
    • Related Report
      2016 Research-status Report
  • [Presentation] 生体高分子の新規リン酸化技術による再生医療・環境浄化新素材の開発2016

    • Author(s)
      久保木芳徳, 戸倉清一, Mostafizur Rahman, 蔵崎正明, 坂入信夫、滝田裕子, 古澤利武, 八上公利,中石典子,高久田和夫
    • Organizer
      第25回硬組織再生生物学会学術大会
    • Place of Presentation
      日本大学歯学部(東京都千代田区)
    • Year and Date
      2016-08-20
    • Related Report
      2016 Research-status Report
  • [Presentation] チタンと、コラーゲンならびにキチンとの反応2016

    • Author(s)
      久保木芳徳,戸倉清一,藏崎正明,八上公利,古澤利武,滝田裕子,Rachel Sammons
    • Organizer
      第48回日本結合組織学会
    • Place of Presentation
      長崎大学医学部(長崎県長崎市)
    • Year and Date
      2016-06-24
    • Related Report
      2016 Research-status Report
  • [Presentation] : コラ―ゲンとチタンの結合:クロマトグラフィーおよび形態的観察による実証2016

    • Author(s)
      久保木 芳徳,戸倉 清一, 藏崎正明
    • Organizer
      第28回代用臓器・再生医学研究会
    • Place of Presentation
      北海道大学工学部フロンティア応用科学研究棟 鈴木章ホール(札幌市)
    • Year and Date
      2016-02-27
    • Related Report
      2015 Research-status Report
  • [Presentation] :生化学的立場から、なぜチタンは骨に付くのか:「生きた骨と金属チタンとの強固な結合」の解明、2015

    • Author(s)
      久保木芳徳、古澤利武、八上公利
    • Organizer
      日本口腔インプラント学会第35回東北北海道支部学術大会
    • Place of Presentation
      仙台市国際会議場(宮城県仙台市)
    • Year and Date
      2015-11-21
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] チタンに結合しインプラントを促進する蛋白の生化学的同定2015

    • Author(s)
      八上公利、古沢利武、村田勝、久保木芳徳
    • Organizer
      日本口腔インプラント学会第35回東北北海道支部学術大会
    • Place of Presentation
      仙台市国際会議場(宮城県仙台市)
    • Year and Date
      2015-11-21
    • Related Report
      2015 Research-status Report
  • [Presentation] Principle of Nanofiber Application in Regenerative Medicine: Geometry of the Artificial Extracellular Matrix,2015

    • Author(s)
      Yoshinori Kuboki, Seiichi Tokura and Masaaki Kurasaki
    • Organizer
      : International Congress of Nanofibers, (国際ナノファイバー学会)
    • Place of Presentation
      東京工業大学・大岡山キャンパス(東京都目黒区)
    • Year and Date
      2015-10-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] インプラント蛋白(チタンに結合して骨を造成する蛋白)の意義とその後の展開2015

    • Author(s)
      久保木芳徳,古澤利武, 八上公利, 蔵崎正明, 戸倉清一
    • Organizer
      第24回・硬組織再生生物学会
    • Place of Presentation
      大阪歯科大学・天満宮キャンパス(大阪市中央区)
    • Year and Date
      2015-08-21
    • Related Report
      2015 Research-status Report
  • [Presentation] チタンとコラーゲンの反応について: インプラント蛋白発見に基づく展開2015

    • Author(s)
      久保木芳徳、国井沙織、森本康一、古澤利武、八上公利
    • Organizer
      第33回日本骨代謝学会
    • Place of Presentation
      京王プラザホテル(東京都・新宿区)
    • Year and Date
      2015-07-25
    • Related Report
      2015 Research-status Report
  • [Presentation] 硬組織再建におけるナノファイバー応用の原理: 人工細胞外マトリックスの幾何学、2015

    • Author(s)
      久保木芳徳
    • Organizer
      ナノファイバー学会第5回年次大会
    • Place of Presentation
      東京大学 弥生講堂 一条ホール(東京都文京区)
    • Year and Date
      2015-07-06
    • Related Report
      2015 Research-status Report
    • Invited
  • [Patent(Industrial Property Rights)] チタンータンパク質複合体の製造方法2015

    • Inventor(s)
      久保木芳徳、古澤利武
    • Industrial Property Rights Holder
      久保木芳徳、古澤利武
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015
    • Acquisition Date
      2017
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 培養基盤固定冶具及びこれを用いた反重力培養方法2015

    • Inventor(s)
      久保木芳徳、菊池敦紀、西村浩之
    • Industrial Property Rights Holder
      久保木芳徳、株式会社イノアック技術研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015
    • Acquisition Date
      2017
    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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