| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In this study, we have elucidated unique reaction mechanisms of new-type heme-degrading enzymes, MhuD from Mycobacterium tuberculosis and IsdG from Staphylococcus aureus, both of which bind heme in highly distorted conformation. MhuD successively catalyzes mono- and di-oxygenation reactions in a single active site. This is the first discovery of the enzyme that fuses the two distinct reactions, leading to the produce the unique heme catabolites of MhuD. Reaction analysis on IsdG reveals, contrary to previous reports, that the MhuD-type reaction becomes dominant under normal reaction conditions. The HCHO release reported is enhanced by increasing reduction rates. This mechanism may be biologically relevant to regulate the HCHO biosynthesis in S. aureus. Furthermore, fluorogenic proteins that bind new heme catabolites as pigments have been developed mainly for their detection with high sensitivity.
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