Development of partially fluorinated phospholipid bilayer membrane

• Project/Area Number: 15K05558
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Gunma University
• Principal Investigator: Sonoyama Masashi 群馬大学, 大学院理工学府, 教授 (40242242)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: リン脂質 / フッ素化 / 膜タンパク質 / パーフルオロアルキル基 / 脂質二分子膜 / タンパク質ー脂質相互作用 / 相分離 / 平面脂質膜 / 膜タンパク質－脂質相互作用 / 膜タンパク質ー脂質相互作用

Outline of Final Research Achievements

Novel partially fluorinated phospholipids have been developed for structural and functional analysis of membrane proteins in vitro. The partially fluorinated phospholipids showed membrane properties and structure significantly different from the corresponding unfluorinated ones. And it has been demonstrated that a model membrane protein in the partially fluorinated phospholipid bilayer adopts nativelike structure and function.

Research Products

• [Journal Article] Study of Perfluoroalkyl Chain-Specific Band Shift in Infrared Spectra on the Chain Length (2017)
  • Author(s): Shimoaka Takafumi、Sonoyama Masashi、Amii Hideki、Takagi Toshiyuki、Kanamori Toshiyuki、Hasegawa Takeshi
  • Journal Title: The Journal of Physical Chemistry A Volume: 121 Issue: 44 Pages: 8425-8431
  • DOI: 10.1021/acs.jpca.7b08940
  • NAID: 120006364087
  • Peer Reviewed
• [Journal Article] Spontaneous stacking of purple membranes during immobilization with physical cross-linked poly(vinyl alcohol) hydrogel with retaining native-like functionality of bacteriorhodopsin (2017)
  • Author(s): Yokoyama Yasunori、Tanaka Hikaru、Yano Shunsuke、Takahashi Hiroshi、Kikukawa Takashi、Sonoyama Masashi、Takenaka Koshi
  • Journal Title: Journal of Applied Physics Volume: 121 Issue: 20 Pages: 204701-204701
  • DOI: 10.1063/1.4984070
  • Peer Reviewed
• [Journal Article] Lateral Diffusion and Molecular Interaction in a Bilayer Membrane Consisting of Partially Fluorinated Phospholipids (2016)
  • Author(s): Y. Okamoto, T. Motegi, K. Morita, T. Takagi, H. Amii, T. Kanamori, M. Sonoyama, R. Tero
  • Journal Title: Langmuir Volume: 32 Issue: 41 Pages: 10712-10718
  • DOI: 10.1021/acs.langmuir.6b02874
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Surface Properties of a Single Perfluoroalkyl Group on Water Surfaces Studied by Surface Potential Measurements (2016)
  • Author(s): Takafumi Shimoaka, Yuki Tanaka, Nobutaka Shioya, Kohei Morita, Masashi Sonoyama, Hideki Amii, Toshiyuki Takagi, Takeshi Hasegawa
  • Journal Title: J. Colloid Interface Sci. Volume: 483 Pages: 353-359
  • DOI: 10.1016/j.jcis.2016.08.055
  • Peer Reviewed
• [Journal Article] Evaluation of diacylphospholipids as boundary lipids for bacteriorhodopsin from structural and functional aspects (2016)
  • Author(s): Kawatake, S., Umekawa, Y., Matsuoka, S., Murata, M., Sonoyama, M.
  • Journal Title: Biochim. Biophys. Acta Volume: 1858 Issue: 9 Pages: 2106-2115
  • DOI: 10.1016/j.bbamem.2016.06.006
  • Peer Reviewed
• [Journal Article] 膜タンパク質研究への展開をめざした部分フッ素化リン脂質膜の開発 (2016)
  • Author(s): 園山正史
  • Journal Title: オレオサイエンス Volume: 16 Pages: 137-143
  • NAID: 130007588007
  • Acknowledgement Compliant
• [Presentation] Membrane Propteries of Partially Fluorinated Amphiphilic Molecules with Different Perfluoroalkyl Chain Lengths (2018)
  • Author(s): Masashi Sonoyama
  • Organizer: Gunma International Symposium 2018
  • Int'l Joint Research / Invited
• [Presentation] Thermotropic Phase Transition Behavior of Partially Fluorinated DipalmitoylphosphatidilcholinesThermotropic Phase Transition Behavior of Partially Fluorinated Dipalmitoylphosphatidilcholines (2018)
  • Author(s): Tamami Yanagi Hiroshi Takahashi Toshinori Motegi Toshiyuki Takagi Hideki Amii Takeshi Hasegawa Toshiyuki Kanamori Masashi Sonoyama
  • Organizer: Gunma International Symposium 2018
  • Int'l Joint Research
• [Presentation] 部分フッ素化dipalmitoylphosphatidylcholineの相転移挙動と二分子膜構造 (2018)
  • Author(s): 柳瑶美 高橋浩 茂木俊憲 高木俊之 網井秀樹 長谷川健 金森敏幸 園山正史
  • Organizer: 第7回日本生物物理学会関東支部会
• [Presentation] 部分フッ素化リン脂質のfluorophilic effectを利用したin vitro膜タンパク質研究の展開 (2017)
  • Author(s): 園山正史
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2017-03-25
  • Invited
• [Presentation] 新規部分フッ素化ジパルミトイルホスファチジルコリンの熱物性におけるパーフルオロアルキル鎖長依存性 (2017)
  • Author(s): 柳瑶美，茂木俊憲，高木俊之，高橋浩，網井秀樹，長谷川健，金森敏幸，園山正史
  • Organizer: 日本化学会 第97春季年会
  • Place of Presentation: 慶応大学日吉キャンパス
  • Year and Date: 2017-03-18
• [Presentation] パーフルオロアルキル基を疎水鎖末端に導入した部分フッ素化リン脂質ライブラリーの開発とin vitro膜タンパク質研究への展開 (2017)
  • Author(s): 園山正史
  • Organizer: フルオラス科学研究会第10回シンポジウム
  • Invited
• [Presentation] 部分フッ素化リン脂質を用いたin vitro膜タンパク質研究の展開 (2017)
  • Author(s): 園山正史 吉野賢 橋本麻美 柳瑶美 茂木俊憲 菊川峰志 高木俊之 高橋浩 網井秀樹 金森敏幸
  • Organizer: 日本ケミカルバイオロジー学会第12回年会
• [Presentation] パーフルオロアルキル鎖長に依存した部分フッ素化リン脂質二分子膜の相転移挙動 (2017)
  • Author(s): 柳瑶美 堀越未希 茂木俊憲 高木俊之 高橋浩 網井秀樹 長谷川健 金森敏幸 園山正史
  • Organizer: 第44回生体分子科学討論会
• [Presentation] 部分フッ素化ジパルミトイルホスファチジルコリンのパーフルオロアルキル鎖長に依存した脂質二分子膜の構造と相転移挙動 (2017)
  • Author(s): 柳瑶美 茂木俊憲 高橋浩 高木俊之 網井秀樹 長谷川健 金森敏幸 園山正史
  • Organizer: 第40回フッ素化学討論会
• [Presentation] 蛍光プローブの回転運動に基づいた部分フッ素化リン脂質二重膜の局所的流動性の評価 (2017)
  • Author(s): 田丸翠允 茂木俊憲 園山正史
  • Organizer: 第68 回コロイドおよび界面化学討論会
• [Presentation] Mixing behaviors in the binary membrane of DMPC and its partially fluorinated analogues with different perfluoroalkyl chain lengths (2016)
  • Author(s): Miki Horikoshi, Kohei Morita, Toshinori Motegi, Hiroshi Takahashi, Hideki Amii, Toshiyuki Takagi, Toshiyuki Kanamori, Masashi Sonoyama,
  • Organizer: 54th Annual Meeting of the Biophyscial Society of Japan(BSj2016)
  • Place of Presentation: Tsukuba International Congress Center(Epochal Tsukuba)
  • Year and Date: 2016-11-26
• [Presentation] Structural stability of bacteriorhodopsin in partially fluorinated analogs of DMPC with different perfluoroalkyl chain lengths (2016)
  • Author(s): Mami Hashimoto, Yuka Murai, Masaru Yoshino, Toshinori Motegi, Takashi Kikukawa, Toshiyuki Takagi, Hiroshi Takahashi, Hideki Amii, Toshiyuki Kanamori, Masashi Sonoyama
  • Organizer: 54th Annual Meeting of the Biophyscial Society of Japan(BSj2016)
  • Place of Presentation: Tsukuba International Congress Center(Epochal Tsukuba)
  • Year and Date: 2016-11-26
• [Presentation] Investigation of fluorescence emission from dye-lipid in partially fluorinated lipid bilayer (2016)
  • Author(s): Toshinori Motegi, Ryugo Tero, Toshiyuki Takagi, Hiroshi Takahashi, Hideki Amii, Toshiyuki Kanamori, Masashi Sonoyama
  • Organizer: 54th Annual Meeting of the Biophyscial Society of Japan(BSj2016)
  • Place of Presentation: Tsukuba International Congress Center(Epochal Tsukuba)
  • Year and Date: 2016-11-25
• [Presentation] Preferential Localization of Bacteriorhodopsin in Binary Lipid Bilayer Composed of Dimyristoylphosphatidylcholine and Its Partially Fluorinated Analog (2016)
  • Author(s): Masashi Sonoyama
  • Organizer: 13th International Conference on Flow Dynamics (ICFD2016)
  • Place of Presentation: Sendai International Center
  • Year and Date: 2016-10-11
  • Int'l Joint Research / Invited
• [Presentation] 新規部分フッ素化リン脂質ライブラリーの構築： 膜タンパク質バクテリオロドプシン研究への応用 (2016)
  • Author(s): 吉野賢，橋本麻美，村井裕佳，森田康平，柳瑶美，茂木俊憲，菊川峰志，高木俊之，高橋浩，網井秀樹，長谷川健，金森敏幸，園山正史
  • Organizer: 第39回フッ素化学討論会
  • Place of Presentation: 佐賀アバンセ
  • Year and Date: 2016-09-30
• [Presentation] 膜タンパク質研究に資する新規部分フッ素化リン脂質ライブラリーの構築： 二本鎖脂質の熱物性におけるパーフルオロアルキル鎖長依存性 (2016)
  • Author(s): 柳瑶美，茂木俊憲，高木俊之，高橋浩，網井秀樹，長谷川健，金森敏幸，園山正史
  • Organizer: 第39回フッ素化学討論会
  • Place of Presentation: 佐賀アバンセ
  • Year and Date: 2016-09-30
• [Presentation] パーフルオロアルキル基を含むリン脂質を用いたin vitro膜タンパク質研究の展開 (2016)
  • Author(s): 園山正史
  • Organizer: 第16回日本蛋白質科学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2016-06-07
  • Invited
• [Presentation] アシル鎖末端をパーフルオロアルキル基で置換 した部分フッ素化ジパルミトイルホスファチジ ルコリンの合成と膜物性の解析 (2016)
  • Author(s): 柳瑶美; 高木俊之; 高橋浩; 網井秀樹; 金森敏幸; 園山正史
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 同志社大学京田辺キャンパス
  • Year and Date: 2016-03-27
• [Presentation] Development of Partially Fluorinated Phospholipids: Physicochemical Characterization of Lipid Bilayer and Reconstituted Membrane Protein Bacteriorhodopsin (2015)
  • Author(s): Sonoyama, M.; Yoshino, M.; Morita, K.; Kanayama, K.; Murai, Y.; Hashimoto, M.; Horikoshi, M.; Takahashi, H.; Amii, H.; Takagi, T.; Kanamori, T.; Kikukawa, T.
  • Organizer: The 5th International Conference on Element Innovation
  • Place of Presentation: Kiryu City Performing Arts Center
  • Year and Date: 2015-11-30
  • Int'l Joint Research
• [Presentation] Structural and Functional Properties of Membrane Protein Bacteriorhodopsin in Partially Fluorinated Phospholipid Bilayer (2015)
  • Author(s): Sonoyama, M.
  • Organizer: The Twelfth International Conference on Flow Dynamics
  • Place of Presentation: Sendai International Center
  • Year and Date: 2015-10-27
  • Int'l Joint Research / Invited
• [Presentation] 疎水鎖末端をパーフルオロアルキル基に置換したDMPC脂質二分子膜中の膜タンパク質の構造・機能・安定性 1. パーフルオロアルキル基によるバクテリオロドプシンの安定化 (2015)
  • Author(s): 橋本麻美; 村井裕佳; 森田康平; 高橋浩; 網井秀樹; 菊川峰志; 高木俊之; 金森敏幸; 園山正史
  • Organizer: 第９回分子科学討論会
  • Place of Presentation: 東京工業大学大岡山キャンパス
  • Year and Date: 2015-09-18
• [Presentation] 疎水鎖末端をパーフルオロアルキル基に置換した DMPC 脂質二分子膜中の 膜タンパク質の構造・機能・安定性 2.DMPC を含む二成分系混合膜におけるバクテリオロドプシンの分配挙動 (2015)
  • Author(s): 堀越未希; 吉野賢; 金山賢治; 森田康平; 高橋浩; 網井秀樹; 菊川峰志; 高木俊之; 金森敏幸; 園山正史
  • Organizer: 第９回分子科学討論会
  • Place of Presentation: 東京工業大学大岡山キャンパス
  • Year and Date: 2015-09-18
• [Presentation] Development of Novel Partially Fluorinated Phospholipid Membrane for Biophysical Studies of Membrane Proteins (2015)
  • Author(s): Sonoyama, M.
  • Organizer: 53回日本生物物理学会年会,
  • Place of Presentation: 金沢大学角間キャンパス
  • Year and Date: 2015-09-14
  • Invited
• [Presentation] 部分フッ素化リン脂質ライブラリーの開発～膜タンパク質の構造・機能解析への応用を目指して～ (2015)
  • Author(s): 園山正史
  • Organizer: 第39回 蛋白質と酵素の構造と機能に関する九州シンポジウム
  • Place of Presentation: 別府豊泉荘
  • Year and Date: 2015-09-10
  • Invited
• [Presentation] 膜タンパク質研究のための部分フッ素化リン脂質ライブラリーの開発 (2015)
  • Author(s): 園山正史; 吉野賢; 村井裕佳; 森田康平; 菊川峰志; 高木俊之; 高橋浩; 網井秀樹; 金森敏幸
  • Organizer: 第14回日本蛋白質科学会年会
  • Place of Presentation: 徳島あわぎんホール
  • Year and Date: 2015-06-26