| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
Development of efficient target identification methods is important for accelerating the discovery of new biological pathways and drug targets. We have designed and synthesized gold-nanoparticle based affinity labeling probes, which display bioactive ligands and photoreactive groups for catch and release of binding proteins in one-pot. Gold nanoparticles are suitable as probe scaffolds due to its capability to covalently immobilize organic compounds in a multivalent fashion, the water dispersibility and the high density, which enable facie centrifugal separation. We demonstrated that our gold-nanoparticle probes can crosslink the binding proteins of high μM affinity with high selectivity at nM probe concentration.
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