Substrate identification method for protein phosphatases through the induction of enzyme-substrate intermediate
| Project/Area Number |
15K05560
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Higashimoto Yuichiro 久留米大学, 医学部, 准教授 (40352124)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ホスファターゼ / リン酸化 / シグナル伝達 / 酵素 / 触媒機構 / がん |
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| Outline of Final Research Achievements |
Protein phosphorylation is strictly regulated by protein kinases and protein phosphatases, and disordered regulation of protein phosphorylation often causes serious disease, such as cancer. It is important to identify substrates for Ser/Thr phosphatases to clarify the signal transduction and disease mechanisms, however, there are still no their reliable methods. Here, we developed two novel substrate-identification methods for Ser/Thr phosphatases, one is method termed Phosphorylation Mimic Phage Display (PMPD), to identify substrate for SCP1 phosphatase using peptide phage display libraries with Mg2+ and AlF4- and another is development of substrate trapping mutants for PPM1D phosphatase. These methods can be useful and applicable for other Ser/Thr phosphatases.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Novel Inhibitors Targeting PPM1D Phosphatase Potently Suppress Cancer Cell Proliferation.2015
Author(s)
Ogasawara, S., Kiyota, Y., Chuman, Y., Kowata, A., Yoshimura, F., Tanino, K., Kamada, R., and Sakaguchi, K.
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Journal Title
Bioorg. Med. Chem.
Volume: 23
Issue: 19
Pages: 6246-6249
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Synthesis of yellow and red fluorescent 1,3a,6a-triazapentalenes and the theoretical investigation of their optical properties2015
Author(s)
Kosuke Namba, Ayumi Osawa, Akira Nakayama, Akane Mera, Fumi Tano, Yoshiro Chuman, Eri Sakuda, Tetsuya Taketsugu, Kazuyasu Sakaguchi, Noboru Kitamura, Keiji Tanino
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Journal Title
Chemical Science
Volume: 6
Issue: 2
Pages: 1083-1093
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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