Pseudo-natural RNA structures as promising modules to construct functional RNA nanostructures with geometrical shapes
| Project/Area Number |
15K05561
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Bio-related chemistry
|
|---|
| Research Institution | University of Toyama |
|---|
Principal Investigator |
Yoshiya Ikawa 富山大学, 大学院理工学研究部(理学), 教授 (70281087)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
FURUTA HIROYUKI 九州大学, 大学院工学研究院, 教授 (40244157)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
|---|
| Keywords | RNAナノテクノロジー / リボザイム / RNA構造 / ナノ集積構造 / RNA / RNAナノ構造 / ナノテクノロジー / RNA工学 |
|---|
| Outline of Final Research Achievements |
RNA is a biopolymer that is attractive for constructing nanoscale objects with complex structures. The 3D structure of a group I (GI) ribozyme has a modular architecture, which can be separated into an activator module and a catalytic module. The fully active ribozyme can be reconstructed by assembling the two separately prepared modules through specific assembly between the two modules. Such non-covalent assembly of the two modules allows the design of 1D-array and polygonal RNA nanostructures. Through rational redesign of the parent GI ribozyme, we constructed variant GI ribozymes as unit RNAs for 1D-arry oligomers and polygonal-shaped oligomers with catalytic activity. Programmed trimerization and tetramerization of the unit RNAs afforded catalytically active nano-sized RNA triangles and squares, the structures of which were directly observed by AFM. We have also seceded in design and construction of RNA nanostructures by modular ribozymes and a fluorescent RNA aptamer.
|
Report
(4 results)
Research Products
(37 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Mutational analysis of structural elements in a class-I cyclic di-GMP riboswitch to elucidate its regulatory mechanism.2016
Author(s)
Inuzuka, S., Nishimura, K., Kakizawa, H., Fujita, Y., Furuta, H., Matsumura, S., Ikawa, Y.
-
Journal Title
The Journal of Biochemistry
Volume: 印刷中
Issue: 3
Pages: 153-162
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
[Journal Article] Prototheca miyajii sp. nov., isolated from a patient with systemic protothecosis2016
Author(s)
Masuda, M., Hirose, N., Ishikawa, T., Ikawa,Y., Nishimura, K.
-
Journal Title
Int. J. Syst. Evol. Microbiol.
Volume: 66
Issue: 3
Pages: 1510-1520
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
