| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
RNA is a biopolymer that is attractive for constructing nanoscale objects with complex structures. The 3D structure of a group I (GI) ribozyme has a modular architecture, which can be separated into an activator module and a catalytic module. The fully active ribozyme can be reconstructed by assembling the two separately prepared modules through specific assembly between the two modules. Such non-covalent assembly of the two modules allows the design of 1D-array and polygonal RNA nanostructures. Through rational redesign of the parent GI ribozyme, we constructed variant GI ribozymes as unit RNAs for 1D-arry oligomers and polygonal-shaped oligomers with catalytic activity. Programmed trimerization and tetramerization of the unit RNAs afforded catalytically active nano-sized RNA triangles and squares, the structures of which were directly observed by AFM. We have also seceded in design and construction of RNA nanostructures by modular ribozymes and a fluorescent RNA aptamer.
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