Development of functional molecules for targeting circulating microRNA
| Project/Area Number |
15K05564
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
Yamayoshi Asako 京都大学, 白眉センター, 特定准教授 (70380532)
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| Co-Investigator(Renkei-kenkyūsha) |
SUGIYAMA Hiroshi 京都大学, 理学研究科, 教授 (50183843)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 核酸医薬 / 機能性分子開発 / microRNA / エクソソーム / 抗体結合型核酸 / miRNA / DDS / アンチセンス核酸 / exosome / 抗体 / 核酸 |
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| Outline of Final Research Achievements |
Exosomes are nano-sized vesicles that are released from various kinds of cells and delivered to the neighboring or distant cells (recipient cells). Such vesicles are widely distributed in various body fluids including blood. Recently, various kinds of nucleic acids including microRNAs (miRNAs) have been identified in exosomes. It has also been reported that such miRNAs in exosomes circulate in the bloodstream and are transported to work at the recipient cells. According to recent reports, the aberrant expression of miRNAs is associated with most pathological disease processes, including carcinogenesis. Therefore, circulating miRNAs are considered as significant therapeutic targets for cancer therapy, however, there is no report to regulate the function of miRNAs in exosomes. In this study, we try to develop novel drug delivery system using cationized antibody for delivery of anti-miR oligonucleotides to the recipient cells and for functional inhibition of circulating miRNAs.
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Report
(4 results)
Research Products
(56 results)
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[Journal Article] Peptide Ribonucleic Acid (PRNA)-Arginine Hybrids. Effects of Arginine Residues Alternatingly Introduced to PRNA Backbone on Aggregation, Cellular Uptake, and Cytotoxicity2018
Author(s)
Sugai, Hiroka; Nakase, Ikuhiko; Sakamoto, Seiji; Nishio, Akihito; Nishijima, Masaki; Yamayoshi, Asako; Araki, Yasuyuki; Ishibashi, Satoru; Yokota, Inoue, Yoshihisa; Wada, Takehiko,*
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Journal Title
Chemistry Letters
Volume: 47
Issue: 3
Pages: 381-384
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Development of Novel Antisense Oligonucleotides for the Functional Regulation2015
Author(s)
Ariyoshi, J., Momokawa, D., Eimori, N., Kobori, A., Murakami A., Yamayoshi, A.
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Journal Title
Bioconjugate Chemistry
Volume: 26
Issue: 12
Pages: 2454-2460
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Hypoxia-Specific oligonucleotide Therapeutics System with Intracellular Environment-Responsive Peptide Ribonucleic Acids (PRNAs)2015
Author(s)
Ryohei Uematsu, Hiroka Sugai, Mitsuo Asai, Masato Inagaki, Seiji Sakamoto, Satoru Ishibashi, Hiromi Kashida, Asako Yamayoshi, Yasuyuki Araki, Hiroyuki Asanuma, Takanori Yokota, Takehiko Wada
Organizer
The 42nd International Synposium on Nucleic Acids Chemistry 2015
Place of Presentation
Eagle Himeji, Hyogo, Japan
Year and Date
2015-09-23
Related Report
Int'l Joint Research
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[Presentation] Gene Silencing by Chemically Modified siRNAs Bearing Cationic Charge on 5’-End2015
Author(s)
Yu Ohno, Kanami Akaike, Yasuhiro Shinkai, Hirohumi Fujii, Ayumi Takashina, Jumpei Ariyoshi, Asako Yamayoshi, Akira Murakami, Masayuki Fujii
Organizer
The 42nd International Synposium on Nucleic Acids Chemistry 2015
Place of Presentation
Eagle Himeji, Hyogo, Japan
Year and Date
2015-09-23
Related Report
Int'l Joint Research
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[Presentation] Gene Silencing by miRNA-like siRNAs Bearing Mismatches and Bulges2015
Author(s)
Yasuhiro Shinkai, Hirohumi Fuji, Yu Ohno, Kanami Akaike, Ayumi Takashina, Jumpei Ariyoshi, Asako Yamayoshi, Akira Murakami, Masayuki Fujii
Organizer
The 42nd International Synposium on Nucleic Acids Chemistry 2015
Place of Presentation
Eagle Himeji, Hyogo, Japan
Year and Date
2015-09-23
Related Report
Int'l Joint Research
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