| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The site specific modification of proteins such as post-translational modification (PTM) is important for studies of protein functions. The PTMs of histones play an important role in the epigenetic regulation. In this research we aimed to develop the methods for preparation of the site specifically modified histones by using the CPE ligation method.
At first, a semi-synthetic method was developed, in which recombinant C-terminal peptides were ligated with the N-terminal site specifically modified CPE peptides, followed by desulfurized to produce trimethyl lysine containing histones H3 and H4. We also developed a convenient method for preparation of isopeptide mimetics by using a thiirane linker. Using this method, ubiquitinated histone H3 peptide and full length H3 were prepared, and these peptides were used for their structural and functional studies. In addition, in order to develop more convenient synthetic method for histones preliminary result of solid phase peptide ligation.
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