Elucidating electron transfer mechanism of new [NiFe]hydrogenase surpassing platinum catalyst
| Project/Area Number |
15K05566
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
Ki-Seok Yoon 九州大学, カーボンニュートラル・エネルギー国際研究所, 准教授 (50701497)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 水素酸化反応 / ヒドロゲナーゼ / 酸素耐性酵素 / 燃料電池 / 電子伝達系 / ゲノム解析 / タンパク質結晶解析 / 生化学的な特性解析 / 水素酸化活性 / 酸素安定性 / 生化学的な特性 / 電子伝達 / タンパク質結晶構造解析 / 水素燃料電池 |
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| Outline of Final Research Achievements |
[NiFe]hydrogenases catalyze the reversible reaction of the cleavage and production of H2, harboring a NiFe center for H2 activation in a large subunit and iron-sulfur clusters for electron transfer in a small subunit. The H2-catalyzing biocatalysts have received much attention due to their potential applications in H2-based fuel cell technology. However, most hydrogenases are highly sensitive to O2, leading to inactivation of catalytic activity after oxidative stress. We recently found the new O2-tolerant [NiFe]hydrogenases from our isolated enterobacterium Citrobacter sp. S-77. The newly found [NiFe]hydrogenase displays an excellent performance for catalytic H2-activation as an O2-tolerant catalyst.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Structural basis of the redox switches in the NAD+-reducing soluble [NiFe]-hydrogenase2017
Author(s)
Y. Shomura, M, Taketa, H. Nakashima, H. Tai, H. Nakagawa, Y. Ikeda, Y, M. Ishii, Y. Igarashi, H. Nishihara, K.-S. Yoon, S. Ogo, Y. Higuchi
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Journal Title
Science
Volume: 537
Issue: 6354
Pages: 928-932
DOI
Related Report
Peer Reviewed
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[Journal Article] A Fusion of Biomimetic Fuel and Solar Cells Based on Hydrogenase, Photosystem II, and Cytochrome c Oxidase.2017
Author(s)
Kikkawa, Mitsuhiro; Yatabe, Takeshi; Matsumoto, Takahiro; Yoon, Ki-Seok; Suzuki, Kazuhau; Enomoto, Takao; Kaneko, Kenji; Ogo, Seiji.
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Journal Title
ChemCatChem
Volume: 9
Issue: 21
Pages: 4024-4028
DOI
Related Report
Peer Reviewed
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[Journal Article] Structural Differences of Oxidized Iron-Sulfur and Nickel-Iron Cofactors in O2-Tolerant and O2-Sensitive Hydrogenases Studied by X-ray Absorption Spectroscopy2015
Author(s)
Sigfridsson, Kajsa G. V.; Leidel, Nils; Sanganas, Oliver; Chernev, Petko; Lenz, Oliver; Yoon, Ki-Seok; Nishihara, Hirofumi; Parkin, Alison; Armstrong, Fraser A.; Dementin, Sébastien; Rousset, Marc; L. De Lacey, Antonio; Haumann, Michael
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Journal Title
Biochim. Biophys. Acta
Volume: 1847
Issue: 2
Pages: 162-170
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Crystallization and preliminary X-ray analysis of the NAD+-reducing [NiFe] hydrogenase from Hydrogenophilus thermoluteolus TH-1.2015
Author(s)
Taketa M, Nakagawa H, Habukawa M, Osuka H, Kihira K, Komori H, Shibata N, Ishii M, Igarashi Y, Nishihara H, Yoon KS, Ogo S, Shomura Y, Higuchi Y.
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Journal Title
Acta Crystallogr F Struct Biol Commun.
Volume: 71
Issue: 1
Pages: 96-99
DOI
Related Report
Peer Reviewed
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