| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
[NiFe]hydrogenases catalyze the reversible reaction of the cleavage and production of H2, harboring a NiFe center for H2 activation in a large subunit and iron-sulfur clusters for electron transfer in a small subunit. The H2-catalyzing biocatalysts have received much attention due to their potential applications in H2-based fuel cell technology. However, most hydrogenases are highly sensitive to O2, leading to inactivation of catalytic activity after oxidative stress. We recently found the new O2-tolerant [NiFe]hydrogenases from our isolated enterobacterium Citrobacter sp. S-77. The newly found [NiFe]hydrogenase displays an excellent performance for catalytic H2-activation as an O2-tolerant catalyst.
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