| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
DNA methylation is involved in the regulation of many epigenetic processes, including embryonic development, cellular differentiation, the etiopathogenesis of tumorigenesis and aging. Demethylation of DNA contributes to cellular reprogramming. Many achievements involving DNA methylation mechanisms have been reported, while, the mechanisms of DNA demethylation is not completely clarified. 5-Hydroxymethylcitosine (5hmC) is regarded as one of the most important intermediates in demethylation processes, however, detection of 5hmC is quite difficult because of its very low abundance.
In this study, an artificial protein/peptide has been developed that bind preferentially to a 5hmC, and interaction between hydroxymethylated DNAs and the protein/peptide was investigated.
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