| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
In this project, we studied the selectivity for the formation of metal-mediated T-Ag(I)-C and T-Hg(II)-T base pairs in DNA polymerase-catalyzed primer extension reaction (PER) by using DNA templates containing the thymine analogs such as 5-fluorouracil (5FU) and 5-hydroxyuracil (OHU), etc. The results indicated that the acidity of NH at the N3 position of thymine is important for the formation of the T-Ag(I)-C base pair in PER and also the stability of DNA duplex containing the T-Ag(I)-C base pair, although the acidity hardly affected on the formation of the T-Hg(II)-T base pair in PER and the stability of DNA duplex containing the T-Hg(II)-T base pair.
Thus, selective regulation of the formation of the T-Ag(I)-C base pair in PER can be successfully achieved by changing the acidity of NH at the N3 position by modifying the 5-methyl group of thymine.
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