Regulation of DNA polymerase-catalyzed primer extension reaction based on formation of metal-mediated base pairs
| Project/Area Number |
15K05574
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Osaka University of Pharmaceutical Sciences |
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Principal Investigator |
Urata Hidehito 大阪薬科大学, 薬学部, 教授(移行) (80211085)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 金属錯体型塩基対 / DNAポリメラーゼ / プライマー伸長反応 / 銀(I)イオン / 水銀(II)イオン / Ag(I)イオン / Hg(II)イオン / ５位修飾ピリミジン塩基 |
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| Outline of Final Research Achievements |
In this project, we studied the selectivity for the formation of metal-mediated T-Ag(I)-C and T-Hg(II)-T base pairs in DNA polymerase-catalyzed primer extension reaction (PER) by using DNA templates containing the thymine analogs such as 5-fluorouracil (5FU) and 5-hydroxyuracil (OHU), etc. The results indicated that the acidity of NH at the N3 position of thymine is important for the formation of the T-Ag(I)-C base pair in PER and also the stability of DNA duplex containing the T-Ag(I)-C base pair, although the acidity hardly affected on the formation of the T-Hg(II)-T base pair in PER and the stability of DNA duplex containing the T-Hg(II)-T base pair.
Thus, selective regulation of the formation of the T-Ag(I)-C base pair in PER can be successfully achieved by changing the acidity of NH at the N3 position by modifying the 5-methyl group of thymine.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Structures, physicochemical properties, and applications of T-HgII-T, C-AgI-C, and other metallo-base-pairs.2015
Author(s)
Yoshiyuki Tanaka, Jiro Kondo, Vladimir Sychrovsky, Jakub Sebera, Takenori Dairaku, Hisao Saneyoshi, Hidehito Urata, Hidetaka Torigoe and Akira Ono
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Journal Title
Chem. Commun.
Volume: 51
Issue: 98
Pages: 17313-17360
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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