Project/Area Number |
15K05657
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Inorganic industrial materials
|
Research Institution | Fukuoka Institute of Technology |
Principal Investigator |
|
Co-Investigator(Renkei-kenkyūsha) |
Hiroyuki Asanuma 名古屋大学, 工学研究科, 教授 (20282577)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
|
Keywords | ナノシート / DNA / ssDNA修飾ナノシート / ハイブリダイゼーション / 消光 / シリル化法 / ホスファイト法 / 六ニオブ酸塩 / 液晶 |
Outline of Final Research Achievements |
Next, we tried to modify the nanosheet surface with ssDNA labeled with a fluorescence molecule. In the fluorescence microscopy observation, fluorescence image was obtained only when the chemical modification was conducted, indicating the fixation of the DNA on the nanosheet. When we added ssDNA, which has complementary base sequence and the quencher molecule, the fluorescence was quenched in 15 min. This indicates that the hybridization reaction between the ssDNAs effectively takes place even at the anomalous nanospace on the nanosheet. DNA/nanosheet colloid system was also investigated. When we added a small amount of DNA to clay nanosheet colloids in isotropic phase, strong liquid crystal phase was obtained.
|