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Biofluorescence measurement micro system by optimization of photonic crystal structure

Research Project

Project/Area Number 15K05715
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Production engineering/Processing studies
Research InstitutionTokyo Institute of Technology

Principal Investigator

YANAGIDA Yasuko  東京工業大学, 科学技術創成研究院, 教授 (10282849)

Co-Investigator(Kenkyū-buntansha) 初澤 毅  東京工業大学, 科学技術創成研究院, 教授 (70272721)
朴 チョンホ (PARK Jongho)  東京工業大学, 科学技術創成研究院, 助教 (80639146)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsマイクロ加工 / フォトニック結晶構造 / バイオ計測 / マイクロシステム / ナノマイクロ加工 / 光導波解析
Outline of Final Research Achievements

Fluorescence assay is well established method for a biochemical sensing for detecting and measuring biological molecules, such as DNA assay. However, light intensity from fluorophores are relatively-weak. In this study, we fabricated a Photonic Cystal (PC) slab for the enhancement of a fluorescence intensity. The PC was made of Si3N4 on a glass substrate and it have a square-lattice hole array. We also fabricated another structure with different lattice constants and hole diameters. We then evaluated the fabricated PhC by introducing the light with the wavelength of 520 nm from the side and measuring the spectrum of emitted light. Moreover, we fabricated a fluorescence measurement device which can arbitrarily change nano periodic structure of photonic crystal by using temperature responsive gel. By transferring nanostructures to a thermoresponsive gel by nanoimprint method, a device was able to change its measurement wavelength arbitrarily with temperature change.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (29 results)

All 2018 2017 2016 2015 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (20 results) (of which Int'l Joint Research: 9 results,  Invited: 1 results) Remarks (4 results)

  • [Journal Article] Development of a Cartridge-type Cell Culture Device Combined with Poly(N-isopropylacrylamide) Coated Substrates2017

    • Author(s)
      Y. Kinoshita, J.G. Park, Y. Yanagida, and T. Hatsuzawa
    • Journal Title

      IEEJ Transactions on Sensors and Micromachines

      Volume: 137 Issue: 3 Pages: 78-83

    • DOI

      10.1541/ieejsmas.137.78

    • NAID

      130005398000

    • ISSN
      1341-8939, 1347-5525
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Micro-rotary ratchets driven by migratory phytoplankton with phototactic stimulus2017

    • Author(s)
      T. Hatsuzawa, D. Ito, T. Nisisako, Y. Yanagida
    • Journal Title

      Precision Engineering

      Volume: 48 Pages: 107-113

    • DOI

      10.1016/j.precisioneng.2016.11.010

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fabrication of p-type porous silicon using double tank electrochemical cell with halogen and LED light sources2016

    • Author(s)
      J.G. Park, Y. Yanagida, and T. Hatsuzawa
    • Journal Title

      Sensors and Actuators B Chemical

      Volume: 233 Pages: 136-143

    • DOI

      10.1016/j.snb.2016.04.058

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Separation of viable and nonviable mammalian cells using a deterministic lateral displacement microfluidic device2016

    • Author(s)
      Naotomo Tottori, Takashi Nishisako, Jongho Park, Yasuko Yanagida, and Takeshi Hatsuzawa
    • Journal Title

      Biomicrofluidics

      Volume: 10 Issue: 1 Pages: 014125-014125

    • DOI

      10.1063/1.4942948

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Design and evaluation of Artemia-driven micro ratchet gears2015

    • Author(s)
      T.Hatsuzawa, A.Yamazaki, T.Nisisako and Y.Yanagida
    • Journal Title

      Sensors and Actuators A: Physical

      Volume: 235 Pages: 182-186

    • DOI

      10.1016/j.sna.2015.10.011

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ナノインプリント法を用いた温度応答性フォトニック結晶の作製2018

    • Author(s)
      櫻井 友紀也, 朴 鍾淏, 初澤 毅, 栁田 保子
    • Organizer
      第65回応用物理学会春季学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ナノビーズ微細構造膜の製作と分光への応用2018

    • Author(s)
      三野 恵莉子, 初澤 毅, 栁田 保子, 朴 鍾淏
    • Organizer
      2018年度精密工学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] バイオ計測のためのマイクロ・ナノデバイスの開発2017

    • Author(s)
      栁田 保子
    • Organizer
      電気化学会第84回大会
    • Place of Presentation
      首都大学東京
    • Year and Date
      2017-03-25
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 33.ガラス基板上のSiN薄膜によるフォトニック結晶構造の光学特性2017

    • Author(s)
      伴野将大,松谷晃宏,Jongho Park,栁田保子,初澤毅
    • Organizer
      第64回応用物理学会春季学術講演会
    • Place of Presentation
      横浜みなとみらいホール
    • Year and Date
      2017-03-14
    • Related Report
      2016 Research-status Report
  • [Presentation] 温度応答性高分子を用いたフォトニック結晶の作製2017

    • Author(s)
      櫻井 友紀也, 朴 鍾淏, 初澤 毅, 栁田 保子
    • Organizer
      2017年度精密工学会秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 裏面露光による電極アレー構造の製作(第2報)熱処理による金薄膜の密着性向上2017

    • Author(s)
      黒坂 幹哉, 朴 鍾淏, 栁田 保子, 初澤 毅
    • Organizer
      2017年度精密工学会秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒドロゲルファイバー表面形状形成のためのマイクロノズル作製2017

    • Author(s)
      TAN ZHENG LIN, 松谷 晃宏, 西岡 國生, JONGHO PARK, 初澤 毅, 栁田 保子
    • Organizer
      第78回応用物理学会秋季学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Characterization of double cell system with halogen and LED illumination for fabrication of porous silicon substrates.2016

    • Author(s)
      J.G. Park, Y. Yanagida, T. Hatsuzawa
    • Organizer
      16th International Conference on Precision Engineering (ICPE2016)
    • Place of Presentation
      Hamamatsu, Japan
    • Year and Date
      2016-11-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Microfluidic generation of satellite-free droplets through a deterministic lateral displacement array.2016

    • Author(s)
      N. Tottori, T. Nisisako, Y. Yanagida, T. Hatsuzawa
    • Organizer
      16th International Conference on Precision Engineering (ICPE2016)
    • Place of Presentation
      Hamamatsu, Japan
    • Year and Date
      2016-11-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] In situ separation of main and satellite droplets using a deterministic lateral displacement microfluidic device2016

    • Author(s)
      N. Tottori, T. Nisisako, Y. Yanagida, T. Hatsuzawa
    • Organizer
      The 20th International Conference on Miniaturized Systems for Chemistry and Life Science (MicroTAS2016)
    • Place of Presentation
      Dublin, Ireland
    • Year and Date
      2016-10-09
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Thermally tunable deterministic lateral displacement through hydrogel micro pillar arrays.2016

    • Author(s)
      N. Tottori, Y. Sakurai, T. Nisisako, Y. Yanagida, T. Hatsuzawa,
    • Organizer
      The 20th International Conference on Miniaturized Systems for Chemistry and Life Science (MicroTAS2016)
    • Place of Presentation
      Dublin, Ireland
    • Year and Date
      2016-10-09
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] フォトニック結晶の光学特性と蛍光測定への応用2016

    • Author(s)
      伴野将大,Jongho Park,栁田保子,初澤毅
    • Organizer
      2016年度精密工学会秋季大会
    • Place of Presentation
      茨城大学
    • Year and Date
      2016-09-08
    • Related Report
      2016 Research-status Report
  • [Presentation] Characterization of double tank electrochemical cell for fabrication of porous silicon2016

    • Author(s)
      8.J.G. Park, Y. Yanagida, T. Hatsuzawa
    • Organizer
      the 8th International Symposium on Microchemistry and Microsystems
    • Place of Presentation
      Hong Kong
    • Year and Date
      2016-05-30
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Label-free Viable Cells Separation Technique using Deterministic Lateral Displacement Microfluidic Device2015

    • Author(s)
      Naotomo Tottori, Jongho Park, Yasuko Yanagida, and Takeshi Hatsuzawa
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2015 (Pacifichem2015)
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of On-site Fluorescent Measurement Chip using Photonic Crystal2015

    • Author(s)
      Yasuko Yanagida, Yasunori Imai, Jongho Park, Tatsuro Endo, and Takeshi Hatsuzawa
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2015 (Pacifichem2015)
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of Deterministic Lateral Displacemnet Microfluidic Device for Viable Cell Separation2015

    • Author(s)
      Naotomo Tottori, Jongho Park, Yasuko Yanagida, and Takeshi Hatsuzawa,
    • Organizer
      The 19th International Conference on Mechatronics Technology (ICMT)
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2015-11-27
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Separation of Viable Cells using Deterministic Lateral Displacement Microfluidic Device2015

    • Author(s)
      Naotomo Tottori, Jongho Park, Yasuko Yanagida, and Takeshi Hatsuzawa
    • Organizer
      The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)
    • Place of Presentation
      Gyeongju, Korea
    • Year and Date
      2015-10-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] DLDマイクロ流路デバイスを用いた生死細胞分離2015

    • Author(s)
      鳥取 直友,朴 鍾淏,栁田 保子,初澤 毅
    • Organizer
      2015年度電気化学秋季大会
    • Place of Presentation
      埼玉工業大学(埼玉)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] エピジェネティクス解析のためのマイクロナノ流体デバイス(第2報)2015

    • Author(s)
      竹山 輝,朴 鍾淏,栁田 保子,初澤 毅
    • Organizer
      2015年度精密工学会秋季大会
    • Place of Presentation
      東北大学 川内北キャンパス
    • Year and Date
      2015-09-04
    • Related Report
      2015 Research-status Report
  • [Presentation] DLDを用いた生死細胞分離デバイス2015

    • Author(s)
      鳥取 直友,朴 鍾淏,栁田 保子,初澤 毅
    • Organizer
      2015年度精密工学会秋季大会
    • Place of Presentation
      東北大学 川内北キャンパス
    • Year and Date
      2015-09-04
    • Related Report
      2015 Research-status Report
  • [Remarks] 東京工業大学未来産業技術研究所 初澤・栁田研究室

    • URL

      http://www.pme.pi.titech.ac.jp/

    • Related Report
      2016 Research-status Report
  • [Remarks] 東京工業大学 未来産業技術研究所

    • URL

      http://www.first.iir.titech.ac.jp/

    • Related Report
      2016 Research-status Report
  • [Remarks] 東京工業大学 未来産業技術研究所 初澤・柳田研究室

    • URL

      http://www.pme.pi.titech.ac.jp/

    • Related Report
      2015 Research-status Report
  • [Remarks] 東京工業大学 未来産業技術研究所

    • URL

      http://www.first.iir.titech.ac.jp/index.html

    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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