Study on Hyper Rapid Cooling Technique using Cryogenic Fluid to Living Cells for Regenerative Medicine

• Project/Area Number: 15K05850
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Thermal engineering
• Research Institution: Akita National College of Technology
• Principal Investigator: NOZAWA Masakazu 秋田工業高等専門学校, その他部局等, 准教授 (60447183)
• Research Collaborator: HATAKEYAMA Sho ; SUGIMOTO You ; SASAKI Hiyu ; OHKOBO Wataru
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 急速冷却 / 極低温流体 / 凍結保存 / 沸騰抑制 / 伝熱特性 / 冷却速度 / 液体窒素 / 液体ヘリウム / 温度計測 / 表面性状 / 極低温 / 熱伝導

Outline of Final Research Achievements

The temperature distribution of the simulated living tissue is measured for the improvement of the cooling rate during cryopreservation when the surface condition of the test sample is changed by covering the stainless steel mesh. The variation of the transient temperature with mesh by the directly immersion in the liquid nitrogen is measured. The temperatures on the sample surface and the inside of the sample are measured by use of type T thermocouples. It is confirmed that on the sample surface there is the slightly temperature increase than that in the saturated liquid nitrogen at the atmospheric pressure. It is found by the comparison of the degree of superheat with or without the mesh that the surface temperature of the test sample with the mesh is lower than that without the mesh. On the other hand, the time series variations of the temperature located in the center of the sample does not change with or without the mesh.

Research Products

• [Journal Article] Effect of surface condition to temperature distribution in living tissue during cryopreservation (2017)
  • Author(s): Masakazu Nozawa, Sho Hatakeyama, You Sugimoto and Hiyu Sasaki
  • Journal Title: IOP Conference Series: Materials Science and Engineering, Advances in Cryogenic Engineering Volume: 274 Pages: 1-7
  • DOI: 10.1088/1757-899x/278/1/012124
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 生体組織の凍結保存における冷却速度促進関する検討 (2018)
  • Author(s): 杉本 陽、大久保 航、野澤 正和
  • Organizer: 日本機械学会学生会第48回学生員卒業研究発表講演会
• [Presentation] Effect of surface condition to temperature distribution in living tissue during cryopreservation (2017)
  • Author(s): Masakazu Nozawa, Sho Hatakeyama, You Sugimoto and Hiyu Sasaki
  • Organizer: Cryogenic Engineering Conference 2017
  • Int'l Joint Research
• [Presentation] 凍結保存における模擬生体組織を用いた温度分布の計測 (2017)
  • Author(s): 畠山 翔、杉本 陽、佐々木 斐裕、野澤 正和
  • Organizer: 日本機械学会学生会第47回学生員卒業研究発表講演会，705
• [Presentation] 凍結保存における模擬生体組織を用いた温度分布の計測 (2017)
  • Author(s): 畠山 翔，杉本 陽，佐々木 斐裕， 野澤 正和
  • Organizer: 日本機械学会 東北学生会 第47回学生員卒業研究発表講演会
  • Place of Presentation: 東北学院大学
• [Presentation] BOILING HEAT TRANSFER OF POROUS NICHROME PLATE HEATER IN LIQUID NITROGEN (2016)
  • Author(s): M. Nozawa, S. Takada, S. Saga, Y. Shirahata
  • Organizer: 4th International Forum on Heat Transfer, IFHT2016
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2016-11-02
  • Int'l Joint Research
• [Presentation] 液体窒素プール沸騰における多孔体ニクロムの沸騰曲線 (2016)
  • Author(s): 野澤 正和，高田 卓
  • Organizer: 2016 年度春季（第93回）低温工学・超電導学会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2016-05-30