| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Applications of microbeads dielectrophoresis (DEP) based DNA detection method were investigated. (1) DEP change of microbead by DNA attachment is derived from not only electric charges on DNA but also permittivity of DNA, (2) application on food contamination by detecting pig mitochondrial DNA sensitive , (3) quantitative detection of norovirus gene (10 copies/reaction) in comparing with real-time polymerase chain reaction, and (4) determination of DNA length by measuring frequency dependent conductance change of DNA attached microbeads. These results suggest that the DNA detection can be applied to detect multiple virus target genes.
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