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Study for biomineralization of biofilm formed on carbon steel

Research Project

Project/Area Number 15K06530
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Metal making/Resorce production engineering
Research InstitutionUniversity of Human Arts and Sciences (2017)
Suzuka National College of Technology (2015-2016)

Principal Investigator

Ikegai Hajime  人間総合科学大学, 人間科学部, 教授(移行) (60184389)

Co-Investigator(Kenkyū-buntansha) 平井 信充  鈴鹿工業高等専門学校, その他部局等, 准教授 (50294020)
Co-Investigator(Renkei-kenkyūsha) KANEMATSU Hideyuki  鈴鹿工業高等専門学校, 材料工学科, 教授 (10185952)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords生体鉱物化 / バイオフィルム / 炭素鋼 / 緑膿菌 / 微生物腐食 / 藍鉄鉱 / セラチア・マルセッセンス / Brevundimonas diminuta / 鉄イオン / 細胞外重合物質
Outline of Final Research Achievements

This study aimed to clarify the mechanism of biomineralization, that is, precipitation of vivianite also known as ferrous phosphate in biofilm (BF) of Pseudomonas aeruginosa PAO1 formed on a carbon steel surface. P. aeruginosa BF created oxygen concentration cells, which ferrous ions eluted from the carbon steel surface (microbiologically influenced corrosion, MIC). Consequently, it appeared that vivianite was formed from ferrous ions and phosphate ions contained in culture medium. Iron concentration accumulated in BF was approximately 13% in comparison with a carbon steel (100%). Analysis of synchrotron radiation computed tomography images showed a number of concave traces, which seemed to indicate MIC, along concave area of BF with bumpy construction. It appears that MIC observed in the present study is different from mechanism of MIC caused by a commonly accepted oxygen concentration cell model.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (21 results)

All 2018 2017 2016 2015

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (13 results) (of which Int'l Joint Research: 2 results) Book (4 results)

  • [Journal Article] Biofilm evaluation methods outside body to inside - Problem presentations for the future -2017

    • Author(s)
      Kanematsu, H., Barry, D. M., Ikegai, H., Yoshitake, M., Mizunoe, Y.
    • Journal Title

      Medical Research Archives

      Volume: 5 Pages: 1-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Biofilm Formation Behaviors on Graphene by E. coli and S. epidermidis2017

    • Author(s)
      Hideyuki Kanematsu, M Sato, Kodai Shindo, Dana M. Barry, Nobumitsu Hirai, Akiko Ogawa, Takeshi Kogo, Yuichi Utsumi, Akinobu Yamaguchi, Hajime Ikegai, Yoshimitsu Mizunoe
    • Journal Title

      ECS Transaction

      Volume: 80 Issue: 10 Pages: 1167-1175

    • DOI

      10.1149/08010.1167ecst

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Application of a loop-type laboratory biofilm reactor to the evaluation and polymers such as urinary stents and catheters2016

    • Author(s)
      Hideyuki Kanematsu, Hikonari Kudara, Shun Kanesaki, Takeshi Kogo, Hajime Ikegai, Akiko Ogawa and Nobumitsu Hirai
    • Journal Title

      Materials

      Volume: 9 Issue: 10 Pages: 824-833

    • DOI

      10.3390/ma9100824

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 鉄鋼材料表面に形成された緑膿菌のバイオフィルムは微生物腐食と生体鉱物化を誘導する2015

    • Author(s)
      生貝 初、小林正和、平井信充、戸田裕之、諸星知広、池田 宰、上杉健太朗、黒田大介、兼松秀行
    • Journal Title

      Bacterial Adherence & Biofilm

      Volume: 29 Pages: 91-94

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] In Biofilm Formation Behavior on Polymer Brush Surfaces by E. coli and S. pidermidis2018

    • Author(s)
      Kanematsu, H., Sato, T., Kamijo, T., Honma, S., Oizumi, A., Umeki, S., Ogawa, A., Hirai, N., Kogo, T., Kuroda, D., Ikegai, H., Mizunoe, Y.
    • Organizer
      2018 TMS Annual Meeting & Exhibition, Phoenix, Arizona, the USA, The Minerals, Metals & Materials Society
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 走査型イオン伝導顕微鏡による常在菌バイオフィルム形態観察2017

    • Author(s)
      平井信充、飯田壮葵、生貝 初、兼松秀行、白澤 樹、吉岡正義、江口由祐、岩田 太
    • Organizer
      平成28年度生体医歯工学共同研究拠点成果報告会
    • Place of Presentation
      東京都文京区東京医科歯科大学
    • Year and Date
      2017-03-24
    • Related Report
      2016 Research-status Report
  • [Presentation] 走査型イオン伝導顕微鏡を用いた常在菌バイオフィルム形態観察2017

    • Author(s)
      平井信充、兼松秀行、生貝 初、白澤 樹、江口由祐、岩田 太
    • Organizer
      日本鉄鋼協会第174回秋季講演大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] バイオフィルムによって引き起こされた微生物腐食の機構2017

    • Author(s)
      生貝 初、小林正和、平井信充、兼松秀行、戸田裕之、上杉健太朗
    • Organizer
      日本鉄鋼協会第174回秋季講演大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] バイオフィルム形成評価に用いる試験菌の選定2017

    • Author(s)
      堀内智博、太田知克、土屋 禎、生貝 初
    • Organizer
      日本防菌防黴学会第44回年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] バイオフィルム形成菌2017

    • Author(s)
      生貝 初
    • Organizer
      日本防菌防黴学会第44回年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] SICMによる常在菌バイオフィルム形態観察の試み2016

    • Author(s)
      平井信充、飯田壮葵、吉岡正義、江口由祐、岩田 太、生貝 初、兼松秀行
    • Organizer
      第36回表面科学学術講演会
    • Place of Presentation
      名古屋市熱田区名古屋国際会議場
    • Year and Date
      2016-11-29
    • Related Report
      2016 Research-status Report
  • [Presentation] Trial of in-situ observation on indigenous bacterium biofilm in aqueous solution by means of scanning ion conductive microscopy2016

    • Author(s)
      Nobumitsu Hirai, Soki Iida, Masayoshi Yoshioka, Yusuke Eguchi, Futoshi Iwata, Hajime Ikegai, and Hideyuki Kanematsu
    • Organizer
      International Symposium on Biomedical Engineering
    • Place of Presentation
      Tokyo Medical and Dental University, Tokyo
    • Year and Date
      2016-11-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 緑膿菌のバイオフィルムによって引き起こされた微生物腐食と生体鉱物化2016

    • Author(s)
      生貝 初、小林正和、平井信充、戸田裕之、上杉健太朗、兼松秀行
    • Organizer
      日本防菌防黴学会第43回年次大会
    • Place of Presentation
      大阪府豊中市千里ライフサイエンスセンター
    • Year and Date
      2016-09-26
    • Related Report
      2016 Research-status Report
  • [Presentation] 各種プラスチック基板上へのバイオフィルムの付着仕事評価2016

    • Author(s)
      平井信充、黒木雅人、生貝 初、兼松秀行、幸後 健、小川亜希子
    • Organizer
      日本鉄鋼協会第172回秋期講演大会
    • Place of Presentation
      大阪府豊中市大阪大学
    • Year and Date
      2016-09-21
    • Related Report
      2016 Research-status Report
  • [Presentation] 鉄鋼材料に微生物腐食を引き起こす緑膿菌バイオフィルムの生体鉱物化2015

    • Author(s)
      生貝 初、平井信充、小林正和、戸田裕之、黒田大介、兼松秀行
    • Organizer
      日本鉄鋼協会 第170回秋季講演大会
    • Place of Presentation
      福岡県福岡市九州大学伊都キャンパス
    • Year and Date
      2015-09-16
    • Related Report
      2015 Research-status Report
  • [Presentation] 鉄鋼材料表面に形成された緑膿菌バイオフィルムの生体鉱物化2015

    • Author(s)
      生貝 初、小林正和、平井信充、戸田裕之、諸星知広、池田 宰、上杉健太朗、黒田大介、兼松秀行
    • Organizer
      日本防菌防黴学会 第42回年次大会
    • Place of Presentation
      大阪府豊中市千里ライフサイエンスセンター
    • Year and Date
      2015-09-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 鉄鋼材料表面に形成された緑膿菌のバイオフィルムは微生物腐食と生体鉱物化を誘導する2015

    • Author(s)
      生貝 初、小林正和、平井信充、戸田裕之、諸星知広、池田 宰、上杉健太朗、黒田大介、兼松秀行
    • Organizer
      日本バイオフィルム学会 第29回学術集会
    • Place of Presentation
      愛知県蒲郡市ホテル竹島
    • Year and Date
      2015-07-10
    • Related Report
      2015 Research-status Report
  • [Book] Fundamentals of Nanoparticles Classifications, Synthesis Methods, Properties and Characterization2018

    • Author(s)
      Kanematsu, H.、Sano, K.,Ikegai, H.,Barry, D.M.,Yoshitake, M.,Mizunoe Y.,Tanaka T.
    • Publisher
      Elsevier
    • Related Report
      2017 Annual Research Report
  • [Book] Handbook of Nanofibers2018

    • Author(s)
      Kanematsu, H., Barry, D. M., Ikegai, H., Yoshitake M., Mizunoe Y.
    • Publisher
      Springer Nature
    • ISBN
      9783319536569
    • Related Report
      2017 Annual Research Report
  • [Book] Corrosion control and surface finishing 担当部分Metal ion sensor of pore-forming toxin for environmental evaluation2016

    • Author(s)
      Hajime Ikegai, In Hideyuki Kanematsu and Dana M, Barry (eds)
    • Publisher
      Springer
    • Related Report
      2016 Research-status Report
  • [Book] Biofilm and Materials Science(担当部分 Genomics Approach)2015

    • Author(s)
      Hajime Ikegai, In Hideyuki Kanematsu and Dana M. Barry(eds)
    • Total Pages
      196
    • Publisher
      Springer
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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