In vitro fabrication of vascular capillary network for organ creation

• Project/Area Number: 15K06547
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Properties in chemical engineering process/Transfer operation/Unit operation
• Research Institution: Kagoshima University
• Principal Investigator: TAKEI Takayuki 鹿児島大学, 理工学域工学系, 准教授 (90468059)
• Co-Investigator(Kenkyū-buntansha): 吉田 昌弘 鹿児島大学, 理工学域工学系, 教授 (50315397)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 血管網 / ファイバー / 毛細血管 / 再生医療

Outline of Final Research Achievements

The purpose of this study is to fabricate vascular capillary network in vitro for organ creation. We fabricated capillary network in collagen gel using gelatin microfibers as a template of the capillary network. Perfusion of culture medium into the capillary network enhanced proliferation of organ cells in collagen gel.

Research Products

• [Journal Article] Autoclavable physically-crosslinked chitosan cryogel as a wound dressing (2018)
  • Author(s): Takayuki Takei, So Danjo, Shogo Sakoguchi, Sadao Tanaka, Takuma Yoshinaga, Hiroto Nishimata, Masahiro Yoshida
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 125 Issue: 4 Pages: 490-495
  • DOI: 10.1016/j.jbiosc.2017.10.015
  • NAID: 40021238833
  • Peer Reviewed
• [Journal Article] <i>In Vitro</i> and <i>in Vivo</i> Characterization of Hydroxyapatite/Chitosan-Gluconic Acid Conjugate Scaffolds (2017)
  • Author(s): Takayuki Takei, Kohei Fukumoto, So Danjo,Takuma Yoshinaga, Hiroto Nishimata, Masahiro Yoshida
  • Journal Title: JOURNAL OF CHEMICAL ENGINEERING OF JAPAN Volume: 50 Issue: 7 Pages: 577-582
  • DOI: 10.1252/jcej.16we202
  • NAID: 130005815307
  • ISSN: 0021-9592, 1881-1299
  • Peer Reviewed
• [Journal Article] Toxic Chemical Cross-linker-free Cryosponges Made from Chitosan-Gluconic Acid Conjugate for Chondrocyte Culture (2017)
  • Author(s): Takayuki Takei, Hiroki Yoshitomi, Kohei Fukumoto, So Danjo,Takuma Yoshinaga, Hiroto Nishimata, Masahiro Yoshida
  • Journal Title: JOURNAL OF CHEMICAL ENGINEERING OF JAPAN Volume: 50 Issue: 2 Pages: 142-148
  • DOI: 10.1252/jcej.16we145
  • NAID: 130005241908
  • ISSN: 0021-9592, 1881-1299
  • Peer Reviewed
• [Journal Article] In vitro formation of vascular-like networks using hydrogels (2016)
  • Author(s): Takayuki Takei, Shinji Sakai, Masahiro Yoshida
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 122 Issue: 5 Pages: 519-527
  • DOI: 10.1016/j.jbiosc.2016.03.023
  • NAID: 40021011614
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Vascular-like network prepared using hollow hydrogel microfibers (2016)
  • Author(s): Takayuki Takei, Zyunpei Kitazono, Yoshihiro Ozuno, Takuma Yoshinaga, Hiroto Nishimata, Masahiro Yoshida
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 121 Issue: 3 Pages: 336-340
  • DOI: 10.1016/j.jbiosc.2015.06.018
  • NAID: 110010051488
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Development of blood capillary-like channel networks fabricated using cotton candy-like gelatin fibers as template of channels (2016)
  • Author(s): Yusuke Yanagi, Takayuki Takei, Yoshihiro Ozuno, Masahiro Yoshida
  • Organizer: The 29th International Symposium on Chemical Engineering
  • Place of Presentation: フェニックスシーガイアリゾート（宮崎県宮崎市）
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] 綿飴状ゼラチンファイバーを用いた毛細血管網再現法の開発 (2016)
  • Author(s): 柳雄介, 武井孝行, 大角義浩, 吉田昌弘
  • Organizer: 日本バイオマテリアル学会シンポジウム2016
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2016-11-21
• [Presentation] Capillary-like network fabricated using “cotton candy”-like gelatin microfibers (2016)
  • Author(s): Yusuke Yanagi, Takayuki Takei, Yoshihiro Ozuno, Masahiro Yoshida
  • Organizer: 22nd Symposium of Young Asian Biochemical Engineers' Community
  • Place of Presentation: フェニックスシーガイアリゾート（宮崎県宮崎市）
  • Year and Date: 2016-10-27
  • Int'l Joint Research
• [Presentation] マイクロゼラチンファイバーを用いた毛細血管網再現法の開発 (2016)
  • Author(s): 柳雄介, 武井孝行, 大角義浩, 吉田昌弘
  • Organizer: 第27回九州地区若手ケミカルエンジニア討論会
  • Place of Presentation: 休暇村志賀島（福岡県福岡市）
  • Year and Date: 2016-07-22
• [Presentation] 綿飴状ゼラチンファイバーを血管の鋳型とする毛細血管網模倣技術の開発 (2016)
  • Author(s): 柳雄介, 武井孝行, 大角義浩, 吉田昌弘
  • Organizer: 第53回化学関連支部合同九州大会
  • Place of Presentation: 北九州国際会議場（福岡県北九州市）
• [Presentation] 中空ゲルファイバーを利用した生体毛細血管網の工学的模倣技術 (2015)
  • Author(s): 武井孝行, 北園純平, 大角義浩, 吉永拓真, 西俣寛人, 吉田昌弘
  • Organizer: 第67回日本生物工学会大会
  • Place of Presentation: 城山観光ホテル（鹿児島県・鹿児島市）
  • Year and Date: 2015-10-26
• [Presentation] Vascular-like microchannel network prepared using hollow hydrogel microfibers (2015)
  • Author(s): Takayuki Takei, Junpei Kitazono, Yoshihiro Ozuno, Takuma Yoshinaga, Hiroto Nishimata, Masahiro Yoshida
  • Organizer: 21st Symposium of Young Asian Biochemical Engineers' Community
  • Place of Presentation: 春川市（韓国）
  • Year and Date: 2015-10-14
  • Int'l Joint Research
• [Presentation] Toxic chemical crosslinker-free chitosan cryosponge for cartilage tissue engineering (2015)
  • Author(s): Takayuki Takei, Hiroki Yoshitomi, Kohei Fukumoto, Yoshihiro Ozuno, Masahiro Yoshida
  • Organizer: 27th European Conference on Biomaterials
  • Place of Presentation: クラコフ（ポーランド）
  • Year and Date: 2015-08-30
  • Int'l Joint Research