| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we investigated the induction of the biological function of Aspergillus niger by pulsed electric field (PEF) stimulation. In particular, the profiles of growth and citric acid fermentation were focused on. All PEF-treated A. niger cultures showed significant increases in mycelial clump size and dry cell weight. These increases changed the profiles of glucose consumption and citric acid production in citric acid fermentation. Combining PEF stimulation with fed-batch citric acid fermentation increases citric acid productivity. The fermentation times to reach the maximum citric acid concentration in the fermentation of PEF-treated and non-PEF-treated A. niger were 16 and 29 days, respectively. Moreover, the volumetric citric acid production rates in the fermentation of PEF-treated and non-PEF-treated A. niger were 1.67 and 0.96 g l-1 day-1, respectively.
|
