| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We created multi-functional caged proteins by modifying sterically bulky protection groups through stimuli-responsive cleavable linkers. Modification of a hydrophilic polymer as the protection group led to strongly stabilize the proteins, so that the modified protein could avoid aggregation even when they were heated. Furthermore, the modified protein was confirmed to drastically recover the activity via light-induced release of the protection group. This molecular technology is a promising tool for stabilizing protein drugs during their storage and transportation. In addition, protein aggregation through a sterically bulky protection group was serendipitously found, and the photolytic protein nano-aggregates were applied to protein regulation and delivery. Moreover, we obtained the preliminary results of both the reductant-responsive and ultrasonic-responsive linkers for expanding the responsibility of the present caged proteins to other external stimuli.
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